Related Experiment Video

Updated: Sep 19, 2025

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.5K

m6A reader IGF2BP2-stabilized lncRNA LHX1-DT inhibits renal cell carcinoma (RCC) cell proliferation and invasion by

Chunming Zhu1, Ruiming Li2, Xiangyun You2,3,4

  • 1Department of Family Medicine, Shengjing Hospital of China Medical University, Shenyang, China.

NPJ Precision Oncology
|June 17, 2025
PubMed

Insights

N6-methyladenosine (m6A) modification regulates renal cell carcinoma (RCC). Long non-coding RNA LHX1-DT, stabilized by IGF2BP2, inhibits RCC progression and serves as a prognostic biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • N6-methyladenosine (m6A) is a critical regulator in human cancers.
  • The role of m6A modification and long non-coding RNA LHX1-DT in renal cell carcinoma (RCC) is not fully understood.

Purpose of the Study:

  • To investigate the role of m6A modification and LHX1-DT in RCC.
  • To elucidate the interaction between IGF2BP2 and LHX1-DT in RCC progression.

Main Methods:

  • Microarray analysis to identify differentially expressed lncRNAs and m6A levels.
  • RNA immunoprecipitation and luciferase reporter assays to examine protein-RNA interactions.
  • Functional assays (e.g., cell proliferation, invasion) to assess the impact of LHX1-DT.

Main Results:

  • LHX1-DT was downregulated in RCC tissues and associated with poor prognosis.
  • Overexpression of LHX1-DT inhibited RCC cell proliferation and invasion.
  • IGF2BP2 recognized m6A on LHX1-DT, enhancing its stability.
  • LHX1-DT sponged miR-590-5p, downregulating PDCD4 and inhibiting RCC progression.

Conclusions:

  • LHX1-DT is an independent prognostic biomarker for RCC.
  • The IGF2BP2/LHX1-DT/miR-590-5p/PDCD4 axis is a potential therapeutic target for RCC.

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.0K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K