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Onsite rapid detection method for genetically modified maize and soybean based on recombinase polymerase

Jia-Tong Yan1,2, Guan-Wei Chen1,2, Qing-Mei Miao2

  • 1College of Life Science, Zhejiang Normal University, Jinhua 321004, China.

Yi Chuan = Hereditas
|June 18, 2025
PubMed
Summary

A new rapid detection method using recombinase polymerase amplification (RPA) allows for quick on-site identification of transgenic maize and soybeans. This technique provides accurate results in about 20 minutes, supporting China

Keywords:
RPA reactionfield detectionfluorescence visualizationgenetically modified cropsrapid extraction

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Area of Science:

  • Agricultural Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Commercial planting of transgenic crops in China necessitates rapid on-site detection methods.
  • Existing methods for detecting genetically modified organisms (GMOs) often lack speed and portability.
  • Ensuring the healthy development of China's biological breeding industry requires efficient GMO monitoring.

Purpose of the Study:

  • To develop a rapid, on-site detection method for transgenic maize and soybeans.
  • To overcome the limitations of current on-site GMO detection techniques.
  • To provide technical support for the on-site detection of transgenic crops.

Main Methods:

  • Utilized recombinase polymerase amplification (RPA) for sensitive and specific DNA amplification.
  • Designed and screened specific primers and probes for target transgenic events.
  • Developed rapid DNA extraction protocols for seed and leaf samples, including chlorophyll interference mitigation.
  • Integrated portable equipment for amplification and visual detection.

Main Results:

  • Established a highly specific and sensitive RPA system detecting down to 20 copies per transgenic event.
  • Developed rapid DNA extraction from seeds using nucleic acid release agents.
  • Created a method for leaf DNA extraction that eliminates chlorophyll interference.
  • Constructed a complete on-site detection system yielding results consistent with conventional PCR in approximately 20 minutes.

Conclusions:

  • The developed RPA-based method offers a practical and adaptable solution for on-site transgenic maize and soybean detection.
  • The technique is suitable for field use, requiring minimal equipment.
  • This approach provides valuable technical support for GMO monitoring and serves as a reference for other rapid nucleic acid detection technologies.