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Updated: Sep 19, 2025

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
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Physical Intracellular Delivery Based on Microfluidic Technology.

Chong Ji1, Yuanyuan Song1, Zhiwei Fan1

  • 1Joint Laboratory of Advanced Biomedical Materials (NFU-UGent), Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University (NFU), Nanjing, 210037, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)
|June 18, 2025
PubMed
Summary
This summary is machine-generated.

Microfluidic chips offer a promising physical method for intracellular delivery of biomolecules, overcoming limitations of traditional techniques. This approach enhances precision medicine and cellular therapies by improving delivery efficiency while minimizing cell damage.

Keywords:
intracellular deliverymechanical penetrationmicrofluidic chip, physical methodsplasma membrane permeabilization

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Intracellular delivery of biomolecules is crucial for biomarker detection and cellular immunotherapies.
  • Current methods like carrier-based and chemical membrane disruption have limitations in cell-type specificity and cell damage.
  • Physical membrane disruption methods offer an alternative but face challenges in balancing efficiency and cell viability.

Purpose of the Study:

  • To systematically review microfluidic chip-based physical intracellular delivery methods.
  • To highlight the mechanisms, approaches, applications, advantages, and limitations of these techniques.
  • To discuss future prospects for advanced intracellular delivery strategies.

Main Methods:

  • Review of existing literature on microfluidic chip-based physical intracellular delivery.
  • Analysis of mechanisms including precise control and high throughput.
  • Examination of traditional physical methods (microneedles, electroporation) for comparison.

Main Results:

  • Microfluidic chips provide precise control and high throughput for physical intracellular delivery.
  • These systems show potential to overcome the cell damage-delivery efficiency trade-off.
  • Emerging microfluidic technologies offer a promising alternative to conventional delivery systems.

Conclusions:

  • Microfluidic chip-based physical intracellular delivery is a rapidly advancing field with significant potential.
  • This technology can enhance precision medicine, cell-based therapies, and biological research.
  • Further development of next-generation strategies is expected to broaden applications and improve outcomes.