Jove
Visualize
Contact Us

Related Concept Videos

Mismatch Repair01:20

Mismatch Repair

5.2K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
5.2K
Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cortical development dynamics across autism spectrum disorder mouse models.

Nature·2026
Same author

Incisional hernia after inverted L-shaped incision in open hepatectomy: A topographical analysis and comparison of 2 fascial closure methods.

Surgery·2026
Same author

tRF-3021a, a tRNA-Ala-TGC derived 3' fragment, promotes glioblastoma cell invasion, suppresses apoptosis, and is required for normal levels of protein synthesis.

bioRxiv : the preprint server for biology·2026
Same author

A Heavy Chain Reaction: Unraveling loop extrusion dynamics during VDJ recombination.

Molecular cell·2026
Same author

A Widely Applicable CAD-Based Intraoperative 3D Navigation Method for Hepatopancreatobiliary Surgery.

Annals of surgical oncology·2025
Same author

Somatic hypermutation patterns are shaped by both motif position and sequence grammar.

The EMBO journal·2025
Same journal

Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

Nucleic acids research·2026
Same journal

Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

Nucleic acids research·2026
Same journal

Evolution of the ribosomal exit tunnel through the eyes of the nascent chain.

Nucleic acids research·2026
Same journal

Enhancing the performance and interpretability of epigenetic clocks.

Nucleic acids research·2026
Same journal

FABIAN-variant 2026: improved prediction of the effects of DNA variants on transcription factor binding.

Nucleic acids research·2026
Same journal

Structural and biochemical characterization of Grimontia hollisae thermostable direct hemolysin with DNA reveals first Vibrio hemolysin with nuclease activity.

Nucleic acids research·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 8, 2025

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

3.4K

Specific origin selection and excess functional MCM2-7 loading in ORC-deficient cells.

Yoshiyuki Shibata1, Mihaela Peycheva2, Etsuko Shibata1

  • 1Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, United States.

Nucleic Acids Research
|June 18, 2025
PubMed
Summary
This summary is machine-generated.

The six-subunit origin recognition complex (ORC) is not essential for DNA replication origin specification or MCM2-7 loading in human cancer cells. Specific origins are still used and excess MCM2-7 is loaded even without ORC.

More Related Videos

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
28:15

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Published on: July 28, 2010

12.5K
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

1.8K

Related Experiment Videos

Last Updated: Sep 8, 2025

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

3.4K
Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
28:15

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Published on: July 28, 2010

12.5K
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
10:52

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

1.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The origin recognition complex (ORC) is crucial for initiating DNA replication by loading MCM2-7 onto chromosomes.
  • ORC is thought to play a key role in specifying DNA replication origins.

Purpose of the Study:

  • To investigate the necessity of the six-subunit ORC for origin specification and MCM2-7 loading in human cancer cell lines.
  • To determine if specific origins are maintained and if excess MCM2-7 loading occurs in the absence of ORC subunits.

Main Methods:

  • Engineered human cancer cell lines with deletions of ORC1, ORC2, or ORC5 subunits.
  • Mapped DNA replication origins in these engineered cell lines.
  • Assessed MCM2-7 loading rates during G1 and S phases.

Main Results:

  • Specific DNA replication origins were utilized at similar genomic sites in ORC-deficient cells compared to wild-type cells.
  • GC/TA skewness and simple repeats were found to facilitate, but not be essential for, origin selection without ORC.
  • Excess MCM2-7 was loaded at comparable rates in G1 phase and re-loaded in S phase, indicating licensing of dormant origins and permitting re-replication despite ORC absence.

Conclusions:

  • The six-subunit ORC is not required for origin specification in human cancer cell lines.
  • Excess MCM2-7 loading and licensing of dormant origins occur independently of the six-subunit ORC.
  • These findings challenge the established role of ORC in origin selection and MCM loading.