Framework Nucleic Acid-Programmed Sensing Interface with Densely Monodispersed Probes

The uniform monodispersity of probes anchored on devices is of great value for molecular recognition and ligand binding. However, the uncontrollability of probe interfacial dispersity makes the probe orientations challenging to disperse on a densely monodispersed level. Herein, we report a framework nucleic acid (FNA)-programmed strategy to construct a densely monodispersed nucleic acid recognition interface. The tetrahedral DNA nanostructure (TDN) was used to disperse single-stranded DNA (Ss-DNA) probes with densely isolated recognition sites on the interface. We found that the monodispersed recognition interface exhibited superior sensing performance, including faster hybridization kinetics, higher hybridization efficiency, and higher signal-to-noise ratio (SNR), in comparison to a conventional SsDNA interface. Further, by programming the length of the outstretched nucleic acid sequence, we demonstrated that the densely monodispersed interface exhibited a 12.7-fold higher SNR compared to that of prolonged probes, which were liable to intertwine. We speculate that the FNA-programmed monodispersed recognition interface shows great applications for constructing devices with excellent sensing performance.