Ophicalcitum-Inspired Microspheres as a Topical Hemostatic Agent

Area of Science:

• Biomaterials Science
• Materials Chemistry
• Traditional Chinese Medicine

Background:

• Ophicalcitum, a traditional Chinese medicine mineral, is recognized for its hemostatic properties.
• Calcium carbonate, the primary component of Ophicalcitum, offers biosafety, structural integrity, and cost-effectiveness.
• There is a need for advanced hemostatic materials with enhanced functionalities.

Purpose of the Study:

• To synthesize calcium carbonate microspheres (CCMs) for hemostatic applications.
• To investigate the influence of particle size on the hemostatic efficacy of CCMs.
• To develop multifunctional hemostatic materials by incorporating luteolin for wound healing promotion.

Main Methods:

• Calcium carbonate microspheres were synthesized using ethylene glycol and ultrapure water.
• Luteolin was loaded onto the synthesized calcium carbonate microspheres.
• Hemostatic properties were evaluated, alongside assessments for hemolytic, cytotoxic, and metabolic toxicity.
• The effect of luteolin-loaded CCMs on skin healing was investigated.

Main Results:

• Synthesized calcium carbonate microspheres exhibited excellent hemostatic capabilities.
• Hemostatic performance was found to be independent of microsphere particle size.
• The materials demonstrated no hemolytic, cytotoxic, or metabolic toxic effects.
• Luteolin-loaded CCMs significantly promoted skin healing.

Conclusions:

• Calcium carbonate microspheres are effective and safe hemostatic materials.
• These microspheres offer a promising platform for developing multifunctional hemostatic agents.
• The combination of calcium carbonate microspheres and luteolin presents a novel approach for wound management and hemostasis.