Related Experiment Video

Updated: May 5, 2026

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels
09:21

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

Published on: December 6, 2012

14.3K

A Step-by-Step Guide for Biosynthesis of Recombinant Fusion Antimicrobial Peptide and Release of the Active Peptide

Sivakumar Jeyarajan1,2, Sukumar Ranjith1, Bency Thankappan1,3

  • 1Microbial Biotechnology Laboratory, Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.

Methods in Molecular Biology (Clifton, N.J.)
|June 18, 2025
PubMed

Keywords:
CloningFormic acid cleavageGenetic engineeringProtein expression optimizationSDS-PAGETherapeutic antimicrobial peptidesThioredoxin

More Related Videos

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.3K
Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics DCAF09:39

Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics DCAF

Published on: September 17, 2019

7.0K

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.4K
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
94