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Ultrastructural aspects of cryofixed nerves.

K Meller

    Cell and Tissue Research
    |January 1, 1985
    PubMed
    Summary

    Cryo-fixation reveals the rat optic and trigeminal nerves

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Microscopy

    Background:

    • Understanding the ultrastructure of nerve cells is crucial for neuroscience.
    • Previous studies often relied on chemical fixation, potentially introducing artifacts.

    Purpose of the Study:

    • To examine the ultrastructure of rat optic and trigeminal nerves using cryo-fixation.
    • To investigate the myelin sheath and axoplasm cytoskeleton with high fidelity.

    Main Methods:

    • Cryo-fixation of nerve tissue using a liquid nitrogen-propane jet.
    • Examination of ultrastructure via advanced electron microscopy techniques.

    Main Results:

    • The axoplasm cytoskeleton comprises a neurofilament and microtubule meshwork.
    • A helical arrangement of cross-linkers suggests a basis for axonal transport.
    • Myelin sheath membranes are unfused pairs with globular subunits, not fused lines.

    Conclusions:

    • Cryo-fixation provides a more accurate view of nerve ultrastructure.
    • Myelin sheath structure differs significantly from classical interpretations.
    • The cytoskeleton's helical arrangement may facilitate axonal transport mechanisms.

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