Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test01:22

Effect of Hepatic Disease on Pharmacokinetics: Pathophysiologic Assessment and Liver Function Test

275
In clinical practice, the direct measurement of hepatic blood flow to evaluate liver function presents significant challenges due to the intricate and specialized nature of the necessary techniques. Consequently, healthcare professionals often rely on empirical estimates derived from thorough patient examinations and liver function tests to gauge liver health. Among the tools at their disposal, the Child–Pugh and MELD scoring systems stand out for their ability to categorize and assess...
275

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An oxygen-permeable, stirrer-pump-integrated liver-heart microphysiological system enables time-resolved analysis in PK-PD relationships of metabolism-dependent cardiotoxicity.

NAM journal·2026
Same author

Circulating cardiac troponin T is an early biomarker of cardiomyocyte injury in Myh6-Cre mice.

Scientific reports·2026
Same author

Homologous cell type markers highlight subdivisions of the domestic chick hippocampal formation.

Scientific reports·2026
Same author

TRIC-A Loss Sensitizes the Heart to β-Adrenergic Stress and Drives Cardiomyocyte Death and Fibrosis.

Biomolecules·2026
Same author

Genome edited intestine liver on a chip system for integrated intestinal hepatic drug absorption and metabolism evaluation.

Scientific reports·2025
Same author

TRIC-A Facilitates Sarcoplasmic Reticulum-Mitochondrial Ca<sup>2+</sup> Signaling Crosstalk in Cardiomyocytes.

Cells·2025

Related Experiment Video

Updated: May 6, 2026

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
08:32

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Published on: March 10, 2017

10.2K

Considerations for developing CYP induction assays in hepatocytes: Insights from a multilaboratory study.

Hiroko Toyoda1, Ayaka Nozue2, Yuki Nishida3

  • 1Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan; Human Emulation System, Central Research Center, Stem Cell Evaluation Technology Research Association (SCETRA), Tsukuba, Japan.

European Journal of Cell Biology
|June 18, 2025
PubMed
Summary

Variability in cytochrome P450 (CYP) induction studies using primary human hepatocytes (PHH) occurred due to inconsistent seeding density and treatment times. Optimizing standard operating procedures (SOPs) is crucial for improving reproducibility in CYP induction assays.

Keywords:
Analytical methodCYP inductionMultilaboratory studyPrimary human hepatocyteStandard operating procedure

More Related Videos

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

9.9K
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
08:05

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Published on: May 6, 2021

3.8K

Related Experiment Videos

Last Updated: May 6, 2026

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
08:32

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Published on: March 10, 2017

10.2K
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

9.9K
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
08:05

An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells

Published on: May 6, 2021

3.8K

Area of Science:

  • Pharmacology
  • Biochemistry
  • Drug Metabolism

Background:

  • Cytochrome P450 (CYP) enzymes are critical for drug metabolism.
  • Accurate CYP induction studies are essential for drug safety assessment.
  • Variability in experimental conditions can impact the reliability of CYP induction data.

Purpose of the Study:

  • To investigate sources of variability in CYP induction studies using primary human hepatocytes (PHH).
  • To identify factors influencing CYP mRNA expression levels.
  • To provide recommendations for improving reproducibility in CYP induction assays.

Main Methods:

  • Multi-laboratory study involving seven laboratories using PHH from a single donor.
  • Standardized procedures for PHH culture and CYP inducer treatment.
  • Analysis of CYP1A2, CYP2B6, and CYP3A4 mRNA expression via collected PHH lysates.
  • Review of laboratory work records and follow-up tests to identify sources of variability.

Main Results:

  • Differences in PHH seeding density and CYP inducer treatment times were identified as key sources of variability.
  • Both seeding density and induction duration significantly influenced CYP mRNA expression levels.
  • Variability in results persisted despite adherence to shared Standard Operating Procedures (SOPs).

Conclusions:

  • Inconsistencies in experimental execution, specifically seeding density and induction duration, contribute to variability in CYP induction studies.
  • Refinement of SOPs and their implementation is critical for enhancing the reproducibility of PHH-based CYP induction assays.
  • Addressing these factors is essential for reliable drug metabolism and safety evaluations.