Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nature-Inspired Trojan Materials as Invisible Enablers of Advanced Humidity Sensors.

Materials (Basel, Switzerland)·2026
Same author

Usability Evaluation and Perceived Performance of the MoonWalking<sup>®</sup> Insole in Safety Footwear.

Sensors (Basel, Switzerland)·2026
Same author

Direct electrochemical immunosensing of tau protein using shape-controlled gold nanoparticles for Alzheimer's diagnostics.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Rehabilitation Service Delivery Models to Foster Healthy Ageing in the WHO European Region: Results From a Cross-Sectional Stakeholder Consultation Involving 45 Countries.

Advances in rehabilitation science and practice·2026
Same author

Disability severity transitions in the community-dwelling Swiss ageing population: secondary analysis of the Swiss version of the Survey of Health, Ageing and Retirement in Europe (SHARE) using multistate models.

BMJ open·2026
Same author

A Portable Insole System for Actively Controlled Offloading of Plantar Pressure for Diabetic Foot Care.

Sensors (Basel, Switzerland)·2025

Related Experiment Video

Updated: Sep 19, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.2K

Chitosan-based molecularly imprinted polymers for point-of-care C-reactive protein detection.

Beatriz Moreira1, Sofia Calvet2, Arcelina Marques3

  • 1BioMark@ISEP, School of Engineering of Polytechnic of Porto, Porto, Portugal; LABBELS/CEB, Centre of Biological Engineering, University of Minho, Braga, Portugal; CIETI-LabRISE, ISEP, Polytechnic of Porto, Porto, Portugal.

Talanta
|June 18, 2025
PubMed
Summary

A novel electrochemical biosensor using chitosan-based molecularly imprinted polymers (MIPs) was developed for sensitive C-reactive protein (CRP) detection. This advanced wound monitoring tool offers potential for point-of-care diagnostics.

Keywords:
BiopolymerBiosensorC-reactive proteinChitosanElectropolymerizationMolecularly imprinted polymers

More Related Videos

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

8.4K
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

10.7K

Related Experiment Videos

Last Updated: Sep 19, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.2K
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

8.4K
Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
08:58

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

10.7K

Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Advanced wound monitoring is crucial for effective first-line therapy.
  • Molecular imprinting technology offers a novel approach for detecting wound inflammation biomarkers.
  • Biopolymeric materials are key components in developing innovative healing solutions.

Purpose of the Study:

  • To develop a highly selective and sensitive electrochemical biosensor for C-reactive protein (CRP) detection.
  • To utilize molecularly imprinted polymers (MIPs) integrated with chitosan for enhanced biosensor performance.
  • To explore the potential of this biosensor for point-of-care (PoC) protein diagnostics in wound management.

Main Methods:

  • Fabrication of a sensor film on gold screen-printed electrodes (Au-SPE) using electropolymerization of aniline in the presence of chitosan and CRP template.
  • Optimization of key parameters including removal agent, scanning rate, and electropolymerization cycles.
  • Characterization of MIP performance through electrochemical response upon template molecule recognition.

Main Results:

  • Optimal sensor performance achieved with five electropolymerization cycles and a scan rate of 50 mV/s.
  • The developed biosensor demonstrated high selectivity and sensitivity for CRP detection.
  • A wide detection range for CRP from 1 pg/mL to 10 ng/mL was established.

Conclusions:

  • Chitosan-based MIPs offer a biocompatible platform for advanced wound monitoring.
  • The biosensor shows significant potential for point-of-care (PoC) protein diagnostics.
  • This approach enhances template stabilization and binding interactions for improved detection capabilities.