Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

342
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
342
Physiological Pharmacokinetic Models: Incorporating Hepatic Transporter-Mediated Clearance01:07

Physiological Pharmacokinetic Models: Incorporating Hepatic Transporter-Mediated Clearance

88
Drug transporters are critical in drug absorption, distribution, and excretion processes. They should be included in physiological-based pharmacokinetic (PBPK) models, which help predict human drug disposition. However, predicting this is challenging during drug development, especially when liver transport is involved. However, with a realistic representation of body transport processes, an accurate model may be possible.
A recent model describes pravastatin's hepatobiliary excretion,...
88
Noncompartmental Analysis: Miscellaneous Pharmacokinetic Parameters00:54

Noncompartmental Analysis: Miscellaneous Pharmacokinetic Parameters

202
The noncompartmental approach is a widely used method in pharmacokinetics to assess drugs' behaviors in the body. It considers several factors, including clearance, bioavailability, and total volume of distribution.
One key aspect of the noncompartmental approach is determining a drug's total clearance. This can be done by dividing the drug dose by the area under the concentration-time curve from zero to infinity. The area under the concentration-time curve represents the drug's...
202
Model Approaches for Pharmacokinetic Data: Distributed Parameter Models01:06

Model Approaches for Pharmacokinetic Data: Distributed Parameter Models

132
Pharmacokinetic models are mathematical constructs that represent and predict the time course of drug concentrations in the body, providing meaningful pharmacokinetic parameters. These models are categorized into compartment, physiological, and distributed parameter models.
The distributed parameter models are specifically designed to account for variations and differences in some drug classes. This model is particularly useful for assessing regional concentrations of anticancer or...
132
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

368
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
368
Pharmacokinetic Models: Overview01:20

Pharmacokinetic Models: Overview

1.2K
Pharmacokinetic models utilize mathematical analysis to achieve a detailed quantitative understanding of a drug's life cycle within the body. They are instrumental in simulating a drug's pharmacokinetic parameters, predicting drug concentrations over time, optimizing dosage regimens, linking concentrations with pharmacologic activity, and estimating potential toxicity.
There are three primary types of models: empirical, compartment, and physiological. Empirical models, with minimal...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cutaneous Bioavailability of Corticosteroids from Topical Formulations: a Retrospective Analysis of Data from In Vitro Permeation Testing (IVPT) and In Vivo Assessments.

Pharmaceutical research·2026
Same author

IVPT Data Challenges in the Real World: Outliers, Anomalous and Aberrant Data: Examples.

Pharmaceutical research·2025
Same author

Bioavailability of Hydroquinone from Topical Formulations: A Product Comparison Study Using the in vitro Permeation Test.

Skin pharmacology and physiology·2025
Same author

Observations on the Tritiated Water and TEWL Skin Integrity Tests: Relevance to In Vitro Permeation Testing (IVPT).

Pharmaceutical research·2024
Same author

Cutaneous Pharmacokinetic Approaches to Compare Bioavailability and/or Bioequivalence for Topical Drug Products.

Dermatologic clinics·2022
Same author

The Tritiated Water Skin Barrier Integrity Test: Considerations for Acceptance Criteria with and Without <sup>14</sup>C-Octanol.

Pharmaceutical research·2016

Related Experiment Video

Updated: Sep 19, 2025

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
11:07

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

2.9K

Using Pharmacokinetic Parameters from in vitro Permeation Test Data for Predicting Multiple-Dose Penetration

Paul A Lehman1

  • 1Independent Scientist, Sumner, Washington, USA.

Skin Pharmacology and Physiology
|June 18, 2025
PubMed
Summary

A simplified pharmacokinetic model predicts skin absorption and barrier content using in vitro permeation test (IVPT) data. This approach estimates topical drug absorption kinetics for multiple doses, aiding formulation and exposure assessments.

Keywords:
IVPTMultiple dosingPercutaneous absorptionPharmacokineticsTopical bioavailability

More Related Videos

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

25.9K
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

25.5K

Related Experiment Videos

Last Updated: Sep 19, 2025

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging
11:07

Visualizing and Quantifying Pharmaceutical Compounds within Skin using Coherent Raman Scattering Imaging

Published on: November 24, 2021

2.9K
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

25.9K
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
10:33

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Published on: February 23, 2018

25.5K

Area of Science:

  • Pharmacokinetics
  • Dermal Drug Delivery
  • Biopharmaceutics

Background:

  • Complex mathematical models exist for percutaneous absorption using IVPT data.
  • A simpler pharmacokinetic approach is proposed for predicting skin absorption and barrier content.

Purpose of the Study:

  • To develop and validate a simplified pharmacokinetic model for predicting percutaneous absorption kinetics.
  • To estimate skin barrier content and permeation profiles after multiple topical applications.

Main Methods:

  • Utilized published and archived in vitro permeation test (IVPT) data.
  • Applied standard single-compartment pharmacokinetic parameters to IVPT data.
  • Derived pharmacokinetic parameters to predict multiple-dose absorption.

Main Results:

  • Pharmacokinetic parameters were derived for various drugs from IVPT data.
  • Predicted multiple-dose absorption kinetics, flux profiles, and skin barrier content.
  • Calculations covered 7-30 day periods with varied dosing intervals (6, 12, 24h).

Conclusions:

  • The model accurately predicts the rate and extent of drug absorption over multiple doses and days.
  • Provides insights for formulation selection and dosing regimen optimization.
  • Enables estimation of skin and systemic exposure levels from topical applications.