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Research Domains Agricultural, Veterinary and Food Sciences Agricultural Biotechnology Agricultural biotechnology diagnostics (incl. biosensors)
Functional characterization of CYP9J26, a cytochrome P450 associated with insecticide resistance in the arbovirus vector Aedes albopictus

Functional characterization of CYP9J26, a cytochrome P450 associated with insecticide resistance in the arbovirus vector Aedes albopictus

Yuhan Zhao ¹, Xiaojie Zhou ², Mei Li ³, Xinghui Qiu ³

Yuhan Zhao ¹, Xiaojie Zhou ², Mei Li ³

¹State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
²State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute of Disinfection and Pest Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China.
³State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

⁰State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.

International Journal of Biological Macromolecules +

|

June 18, 2025

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View abstract on PubMed

Summary

This summary is machine-generated.

The AalbCYP9J26 gene in Aedes albopictus mosquitoes is crucial for metabolizing and resisting insecticides like malathion and permethrin. This finding aids in developing new strategies against mosquito-borne diseases.

Area Of Science

Medical Entomology
Molecular Biology
Biochemistry

Background

Aedes albopictus mosquitoes transmit arboviruses like dengue, chikungunya, and Zika.
Insecticide resistance in Ae. albopictus threatens vector control programs.
The role of AalbCYP9J26 in insecticide metabolism and resistance was previously unknown.

Purpose Of The Study

To investigate the metabolic capacity of AalbCYP9J26 towards common insecticides.
To confirm the role of AalbCYP9J26 in conferring insecticide tolerance.
To explore AalbCYP9J26 as a biomarker and potential target for insecticide resistance management.

Main Methods

Enzyme system reconstituted with E. coli-expressed AalbCYP9J26, AalbCPR, and Aalbb5.
In vitro metabolic assays with six insecticides.
Drosophila GAL4/UAS transgenic system to assess insecticide tolerance.

Main Results

AalbCYP9J26 metabolized temephos, malathion, propoxur, permethrin, and deltamethrin.
No significant activity was observed against bendiocarb.
Enhanced AalbCYP9J26 expression increased tolerance to malathion, permethrin, deltamethrin, and propoxur.

Conclusions

AalbCYP9J26 plays a significant role in insecticide metabolism and resistance in Ae. albopictus.
AalbCYP9J26 is a potential biomarker for monitoring insecticide resistance.
AalbCYP9J26 represents a promising target for developing novel insecticidal agents.

Keywords:

Asian tiger mosquito CYP9J26 Cytochrome P450 monooxygenase Insecticide metabolism insecticide resistance

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