Related Experiment Video
Updated: Sep 19, 2025
09:14
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
4.1K
R9AP is a common receptor for EBV infection in epithelial cells and B cells
Yan Li1,2, Hua Zhang3, Cong Sun1
1State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat-sen University Cancer Center, Guangzhou, China.
Nature
|June 18, 2025
Summary
R9AP is identified as a common receptor for Epstein-Barr virus (EBV) entry into epithelial and B cells. This discovery offers a potential target for new EBV prophylactic and vaccine strategies.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Epstein-Barr virus (EBV) infects over 90% of humans, causing various diseases.
- EBV uses distinct host receptors for epithelial and B cell infection.
- A common receptor for EBV entry into both cell types was previously unknown.
Purpose of the Study:
- To identify the common receptor mediating Epstein-Barr virus (EBV) entry into epithelial and B cells.
- To elucidate the mechanism of EBV-receptor interaction and viral entry.
- To explore R9AP as a potential target for EBV therapeutics.
Main Methods:
- R9AP gene silencing and knockout experiments.
- EBV binding and uptake assays.
- Co-immunoprecipitation and Western blotting to study protein interactions.
- Neutralization assays using antibodies.
Main Results:
- R9AP was established as a crucial receptor for EBV entry into both epithelial and B cells.
- R9AP directly binds the EBV gH/gL complex, initiating membrane fusion.
- Inhibition of R9AP or its interaction with gH/gL blocked EBV infection.
- R9AP cooperates with specific EBV glycoprotein complexes for viral entry.
Conclusions:
- R9AP is the common receptor for EBV entry into epithelial and B cells.
- R9AP represents a promising target for developing EBV prophylactic and vaccine strategies.
- Understanding R9AP's role advances knowledge of EBV pathogenesis and control.
Related Concept Videos
01:03Rous Sarcoma Virus (RSV) and Cancer
5.4K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.4K
01:14Rab Proteins
4.1K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.1K
01:28Diversity of Antigen Receptors
818
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
818
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K