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A Novel Method for Culturing Telencephalic Neurons in Axolotls.
Sevginur Bostan1,2,3, Safiye Serdengeçti1,3,4, F Kemal Bayat5
1Research Institute for Health Sciences and Technologies (SABITA), Regenerative and Restorative Medicine Research Center (REMER), Istanbul Medipol University, Istanbul, Türkiye.
The Journal of Comparative Neurology
|June 19, 2025
Summary
Researchers developed a new method to culture axolotl (Ambystoma mexicanum) brain cells. These functional in vitro models will help study nervous system regeneration and neurodegenerative diseases.
Area of Science:
- Neuroscience
- Regenerative Medicine
- Amphibian Biology
Background:
- The axolotl (Ambystoma mexicanum) is a key model organism for studying nervous system regeneration due to its remarkable regenerative capabilities.
- Understanding the cellular and molecular mechanisms of axolotl regeneration is hindered by the lack of reliable in vitro models for neural cells.
Purpose of the Study:
- To develop a novel protocol for primary cultures of adult axolotl telencephalon/pallium.
- To establish a reliable in vitro system for studying axolotl neurogenesis and regeneration.
Main Methods:
- Primary cell culture of adult axolotl telencephalon/pallium.
- Calcium imaging to assess cellular activity.
- Immunocytochemistry to identify neuronal and glial markers.
Main Results:
- Established viable and functionally active primary cultures of axolotl neural cells.
- Demonstrated the presence of neuronal and glial markers, synaptic connections, and spontaneous calcium activity.
- Showcased robust neurite outgrowth and responsiveness to injury in cultured neurons, maintainable in serum and serum-free conditions.
Conclusions:
- The developed protocol provides a critical in vitro model for axolotl neural research.
- This system facilitates the study of neurogenesis and regeneration in axolotls.
- The findings offer insights into regenerative mechanisms, enabling comparative studies with mammalian systems and potential therapeutic applications for human neurological conditions.
