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Related Experiment Video

Updated: Sep 19, 2025

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Published on: June 15, 2022

In-flow measurement of molecular diffusion coefficients using differential dynamic microscopy.

U Eberhard^1,2, M Usuelli³, E Secchi¹

¹ETH Zurich, Department of Civil, Environmental and Geomatic Engineering, 8093 Zurich, Switzerland.

Physical Review. E

|June 19, 2025

Summary

This summary is machine-generated.

This study introduces a new microscopy technique to measure molecular diffusion in flowing fluids, overcoming challenges posed by sample deformation and varying shear rates for accurate transport studies.

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Area of Science:

Fluid dynamics
Microfluidics
Physical chemistry

Background:

Diffusive mass transport is crucial in fields from medicine to oceanography.
Experimentally measuring diffusion in flowing fluids is difficult due to shear-induced sample deformation.

Purpose of the Study:

To develop a robust method for quantitatively measuring molecular diffusion coefficients in laminar flow profiles.
To address the challenges of local sample deformation and varying shear rates in experimental diffusion studies.

Main Methods:

Differential dynamic microscopy (DDM) combined with image velocimetry.
Utilizing subresolution tracer particles within a rectangular microfluidic channel.
Scanning through different focal planes to capture variations across the flow profile.

Main Results:

Successfully measured local diffusion coefficients across a laminar flow profile.
Quantified diffusion in the presence of varying shear rates.
Demonstrated the method's robustness in a microfluidic setting.

Conclusions:

Differential dynamic microscopy with image velocimetry offers a powerful approach to study diffusion in dynamic fluid environments.
The method is adaptable to complex fluids with shear-dependent viscosity.
Provides a foundation for broader applications in studying transport phenomena.