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Related Experiment Videos

Changes in human plasma alpha-D-mannosidase activity during pregnancy.

S Hirani, B Winchester

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |October 31, 1985
    PubMed
    Summary

    Plasma levels of acidic and intermediate alpha-mannosidase increase during pregnancy. The acidic form shows a 2-3 fold rise, mainly due to a more negatively charged variant, B2.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Reproductive Biology

    Background:

    • Alpha-mannosidase is an enzyme involved in glycoprotein metabolism.
    • Changes in enzyme activity during pregnancy can indicate physiological adaptations.
    • Understanding these changes is crucial for monitoring maternal health.

    Purpose of the Study:

    • To investigate the changes in plasma acidic and intermediate alpha-mannosidase during pregnancy.
    • To characterize the properties of these enzymes in pregnant individuals.

    Main Methods:

    • Enzyme activity assays for acidic and intermediate alpha-mannosidase.
    • Concanavalin A chromatography and gel filtration to analyze enzyme forms.
    • Analysis of charge variants using techniques like isoelectric focusing (implied).

    Main Results:

    • Significant, progressive increases in plasma acidic (pH optimum 4.2) and intermediate (pH optimum 5.6) alpha-mannosidase were observed during pregnancy.
    • Acidic alpha-mannosidase in pregnancy binds to concanavalin A and shares similar molecular weight with controls.
    • The increase in acidic alpha-mannosidase is attributed to a more negatively charged form, B2.
    • Intermediate alpha-mannosidase increased by ~50% and resolved into similar forms as in controls.

    Conclusions:

    • Plasma alpha-mannosidase levels, particularly the acidic form and its B2 variant, are altered during pregnancy.
    • These alterations suggest pregnancy-specific modifications in glycoprotein metabolism.
    • Further research could explore the clinical significance of these enzymatic changes.

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