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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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A MATLAB-based Instrument Control (MIC) package for fluorescence imaging.

Sajjad A Khan1,2, Sandeep Pallikkuth1, David J Schodt1

  • 1Department of Physics and Astronomy, University of New Mexico, Albuquerque, New Mexico, USA.

Journal of Open Source Software
|June 19, 2025
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Summary
This summary is machine-generated.

MATLAB Instrument Control (MIC) software streamlines custom microscope data collection. Its object-oriented design and modular classes enable flexible, automated control for diverse scientific instruments.

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Area of Science:

  • Microscopy and Imaging Systems
  • Scientific Software Development
  • Instrumentation and Control

Background:

  • Custom-built microscopes often require tailored software for data acquisition.
  • Existing solutions may lack flexibility for diverse instrument configurations.
  • Object-oriented programming offers a robust framework for modular instrument control.

Purpose of the Study:

  • To introduce MATLAB Instrument Control (MIC), a software package for custom microscope data collection.
  • To provide a standardized, object-oriented framework for controlling diverse low-level instruments.
  • To enable flexible, user-defined operation modes for interactive and automated data acquisition.

Main Methods:

  • MIC utilizes object-oriented programming with a class for each low-level instrument.
  • Abstract classes and subclasses standardize interfaces for components like lasers, stages, and cameras.
  • End-user development of interoperation allows for customized control sequences and GUIs.

Main Results:

  • MIC facilitates the creation of uniform interfaces across various instruments.
  • The modular design simplifies the development of control classes for new hardware.
  • It supports the construction of both simple and complex data collection systems.

Conclusions:

  • MIC offers a flexible and extensible platform for custom microscope control and data acquisition.
  • The software enables rapid prototyping and the development of high-level user interfaces for production instruments.
  • It empowers researchers to design customized, automated workflows for scientific data collection.