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Escherichia Phage Ge15, NRG-P0073: Genomic Characterization and Host Range Analysis Against the ECOR Reference

Ranee K Anderson1, Shijie Qin1, Rachel M Carson1

  • 1Department of Food Science, Cornell University, Ithaca, NY, USA.

PHAGE (New Rochelle, N.Y.)
|June 19, 2025
PubMed
Summary

We fully sequenced and characterized the Escherichia phage Ge15 (NRG-P0073), revealing its genomic properties and host interactions. Further research is needed to identify its specific receptor-binding protein for potential applications.

Keywords:
ECOR libraryGe15Keio collectionNRG-P0073bacteriophagehost rangephage

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Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophage (phage) characterization is vital for applications in medicine, agriculture, and biotechnology.
  • Understanding phage properties requires genomic annotation, morphology, and host range analysis.
  • This study focuses on Escherichia phage Ge15 (NRG-P0073), an unclassified Tequatrovirus.

Purpose of the Study:

  • To present the fully annotated genome of Escherichia phage Ge15 (NRG-P0073).
  • To characterize the phage's morphology and host range.
  • To identify the receptor-binding protein responsible for host recognition.

Main Methods:

  • Whole genome sequencing, de novo assembly, and annotation using Galaxy and Apollo software.
  • Double-agar spot tests against the E. coli Reference (ECOR) library and Keio collection knockouts.
  • Transmission electron microscopy for phage morphology elucidation.

Main Results:

  • NRG-P0073 possesses a 170,913 bp genome with 259 genes and 35.5% GC content.
  • The phage adsorbed 45.8% of ECOR strains and replicated in 20.8%.
  • All tested E. coli K-12 single-gene knockout strains supported replication, indicating no single evaluated receptor is solely responsible for attachment.

Conclusions:

  • Novel genomic data and characterization of phage NRG-P0073 are presented.
  • Further studies are required to identify the specific E. coli receptor-binding protein.
  • This work provides a foundation for future machine-learning studies on phage-host interactions.