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Ginsenoside Rg3 exerts anticancer effects in lung cancer through metabolite Histon H3
Huiyan Xiao1, Yongcong Zhang2, Banghui Zhang2
1Department of Clean Operating, Quanzhou First Hospital Quanzhou 362002, Fujian, China.
Objectives:
To investigate the effects of ginsenoside Rg3 on metabolites in lung cancer cells.
Methods:
A549 cells were inoculated into nude BALB/c mice. Ginsenoside Rg3 (0.2 mL) or normal saline was orally administered daily for 12 days. LC/MS-based metabolomics was performed to analyze the metabolite profiles across three groups.
Results:
In serum samples, 143 metabolites were significantly different between the model and ginsenoside Rg3 groups. In fecal samples, 44 metabolites differed significantly between the two groups. Levels of Lamin A/C and Histon H3 were upregulated in model tissues. Ginsenoside Rg3 treatment significantly inhibited the expression of Lamin A/C and Histon H3, suggesting inhibition of histidine metabolism activation in lung cancer. Furthermore, ginsenoside Rg3 or Lamin A knockdown inhibited histamine-induced proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) in lung cancer cells.
Conclusions:
Ginsenoside Rg3 significantly altered the metabolic profile in lung cancer mice. Mechanistically, ginsenoside Rg3 downregulated Lamin A/C through histidine metabolic pathway and suppressed histamine-induced progression of lung cancer.
Insights
Ginsenoside Rg3 alters lung cancer cell metabolism, downregulating Lamin A/C and inhibiting histidine metabolism. This natural compound suppresses tumor progression by targeting key pathways, offering a potential therapeutic strategy.
Area of Science:
- Oncology
- Metabolomics
- Pharmacology
Background:
- Lung cancer is a leading cause of cancer death.
- Aberrant metabolism plays a critical role in lung cancer development and progression.
- Ginsenoside Rg3 is a natural compound with potential anti-cancer properties.
Purpose of the Study:
- To investigate the effects of ginsenoside Rg3 on the metabolic profile of lung cancer cells.
- To elucidate the underlying mechanisms by which ginsenoside Rg3 exerts its anti-cancer effects.
Main Methods:
- A549 lung cancer cells were xenografted into nude mice.
- Mice were treated with ginsenoside Rg3 or saline.
- LC/MS-based metabolomics was employed to analyze serum and fecal samples.
Main Results:
- Ginsenoside Rg3 significantly altered metabolite profiles in serum and fecal samples.
- Treatment downregulated Lamin A/C and Histon H3, indicating inhibition of histidine metabolism.
- Ginsenoside Rg3 and Lamin A knockdown suppressed histamine-induced proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
Conclusions:
- Ginsenoside Rg3 effectively modulates the metabolic landscape in lung cancer.
- It suppresses lung cancer progression by downregulating Lamin A/C via the histidine metabolic pathway.
- Ginsenoside Rg3 presents a promising therapeutic agent for lung cancer treatment.
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