Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Asgard archaeal ESCRT-III system forms helical filaments and remodels eukaryotic-like membranes.

The EMBO journal·2025
Same author

Self-assembled active actomyosin gels spontaneously curve and wrinkle similar to biological cells and tissues.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Surface tension between liquids containing antagonistic and regular salts.

The European physical journal. E, Soft matter·2023
Same author

Smart design of universally decorated nanoparticles for drug delivery applications driven by active transport.

The European physical journal. E, Soft matter·2023
Same author

Charge Regulation of Poly(acrylic acid) in Solutions of Non-Charged Polymer and Colloids.

Polymers·2023
Same author

Phase lines in mean-field models with nonuniform external forces.

The Journal of chemical physics·2022

Related Experiment Video

Updated: Sep 8, 2025

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells
10:10

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

Published on: July 3, 2025

479

Strategy for Generating Giant Unilamellar Vesicles with Tunable Size Using the Modified cDICE Method.

Ariel Chen1, Shachar Gat1, Lior Ohana1

  • 1Department of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.

ACS Synthetic Biology
|June 19, 2025
PubMed
Summary

This study optimizes giant unilamellar vesicle (GUV) production for artificial cells by controlling GUV size distribution. Key parameters refine vesicle size, enabling precise construction for synthetic biology applications.

Keywords:
GUVsemulsion-based GUV productionencapsulation efficiencymodified cDICE methodsize selectiontotal GUV yield

More Related Videos

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles
07:48

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: November 10, 2021

4.4K
Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

15.0K

Related Experiment Videos

Last Updated: Sep 8, 2025

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells
10:10

Synthesis of Compound Giant Unilamellar Vesicles: A Biomimetic Model of Nucleate Cells

Published on: July 3, 2025

479
Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles
07:48

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: November 10, 2021

4.4K
Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

15.0K

Area of Science:

  • Biophysics
  • Synthetic Biology
  • Materials Science

Background:

  • Giant unilamellar vesicles (GUVs) are crucial for constructing artificial cells.
  • Current GUV generation methods often yield broad, uncontrolled size distributions.
  • This size variability hinders the development of reproducible artificial cell systems.

Purpose of the Study:

  • To develop an optimization strategy for controlling GUV size distribution.
  • To identify key experimental parameters that influence GUV size.
  • To facilitate the creation of cell-sized compartments for synthetic biology.

Main Methods:

  • Utilized a modified continuous droplet interface crossing encapsulation method for GUV generation.
  • Systematically varied parameters including chamber rotation time, angular frequency, and inner solution density.
  • Employed a physical model to explain observed size selection phenomena.

Main Results:

  • Demonstrated effective refinement of GUV size distribution by adjusting key experimental parameters.
  • Identified specific parameters as practical 'knobs' for precise GUV size control.
  • Confirmed high encapsulation efficiency is maintained across a range of inner solution salinities, including physiological concentrations.

Conclusions:

  • The study presents a practical method for selecting GUV sizes, addressing a critical limitation in artificial cell construction.
  • Optimized GUV production facilitates the creation of cell-sized compartments with desired biological properties.
  • This work advances synthetic biology by enabling more controlled and reproducible artificial cell development.