Protein-Polymer Coassembly Supraparticles as a Polyester-Based Drug Delivery Carrier with Ultrahigh Colloidal...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Drug Delivery Systems

Background:

• Poly(lactic-co-glycolic acid) (PLGA) nanoparticles are widely used for sustained drug release.
• Existing PLGA nanoparticles face challenges with colloidal stability and drug loading capacity.

Purpose of the Study:

• To develop a novel nanoparticle with improved colloidal stability and drug loading.
• To investigate a new method for creating protein-polymer coassembly supraparticles (PPCAS).
• To evaluate the efficacy of PPCAS for sustained drug release and anticancer therapy.

Main Methods:

• Spontaneous coassembly of bovine serum albumin (BSA) and PLGA to form PPCAS.
• Investigated two drug loading methods: coassembly and solvent diffusion.
• Combined loading methods to enhance drug encapsulation efficiency.
• Assessed colloidal stability, drug loading capacity, sustained release profiles, and anticancer efficacy.
• Evaluated scalability of PPCAS production.

Main Results:

• Achieved ultrahigh colloidal stability (>210 days) and drug loading (∼40% for doxorubicin).
• Combined loading methods resulted in higher drug loading than individual methods.
• Demonstrated sustained drug release from PPCAS.
• PPCAS showed potential in anticancer therapy.
• Successful preliminary scale-up of PPCAS production without quality loss.

Conclusions:

• PPCAS represent a promising platform for advanced drug delivery systems.
• The developed nanoparticles overcome limitations of traditional poly(ester) nanoparticles.
• PPCAS offer a scalable and effective solution for sustained drug delivery and cancer treatment.