MIL-53(Fe)-Glucose self-assembled complex for enhanced angiogenesis and endothelial tip cell activation

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Nanotechnology

Background:

• Critical bone defects present significant clinical challenges.
• Effective bone regeneration requires robust vascularization.
• Metal-organic frameworks (MOFs) are promising nanocarriers for therapeutic delivery.

Purpose of the Study:

• To investigate the role of MIL-53(Fe) MOF in bone regeneration-associated neovascularization.
• To elucidate the molecular mechanisms underlying MIL-53(Fe)'s effect on angiogenesis.
• To evaluate MIL-53(Fe) as a nanocarrier for bone defect repair.

Main Methods:

• Synthesis and characterization of MIL-53(Fe) nanocarriers.
• Assessment of MIL-53(Fe) dispersion stability and biocompatibility.
• In vitro evaluation of endothelial cell activation and angiogenesis.
• Investigation of the Hippo/Yes-associated protein (YAP) pathway.

Main Results:

• MIL-53(Fe) demonstrated excellent dispersion stability and biocompatibility.
• MIL-53(Fe) significantly enhanced endothelial tip cell activation and angiogenesis.
• The MIL-53(Fe)@Glucose complex increased endothelial cell glycolytic activity.
• Activation of the Hippo/YAP pathway was linked to enhanced tip cell phenotype.

Conclusions:

• MIL-53(Fe) nanocarriers effectively promote neovascularization in bone regeneration.
• MIL-53(Fe) enhances angiogenesis by increasing endothelial cell glycolytic activity via the Hippo/YAP pathway.
• MIL-53(Fe) shows potential as a therapeutic strategy for repairing critical bone defects.