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Vineyard pruning-wood waste valorisation: sustainable extraction of bioactive compounds

  • 0Department of Drug Sciences, University of Pavia, Pavia, Italy.
Frontiers in Chemistry +

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June 20, 2025

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June 20, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Grapevine pruning waste can be sustainably processed to extract valuable compounds like (E)-resveratrol and (E)-ε-viniferin. This study optimized a low-impact method for isolating these potent phytochemicals.

Area Of Science

  • Agricultural Science
  • Phytochemistry
  • Green Chemistry

Background

  • Rising global population increases waste, necessitating sustainable development and circular economy principles.
  • Vineyard pruning waste is a significant byproduct rich in valuable secondary metabolites.
  • Phytochemicals like (E)-resveratrol and (E)-ε-viniferin possess biological activity and industrial potential.

Purpose Of The Study

  • To develop a low environmental impact procedure for extracting and isolating (E)-resveratrol and (E)-ε-viniferin from grapevine pruning residues.
  • To optimize microwave-assisted solvent extraction (MASE) and subsequent purification techniques.
  • To evaluate the greenness and environmental impact of the developed procedure.

Main Methods

  • Microwave-assisted solvent extraction (MASE) using 100% ethanol.
  • Medium pressure automated chromatography with n-hexane and ethyl acetate gradient elution.
  • Optional preliminary liquid/liquid extraction.
  • Development of a UHPLC-UV/DAD method for quantification.
  • Assessment of method greenness using AGREE calculator and environmental impact factor (EF).

Main Results

  • Optimized MASE protocol: 5 minutes at 80°C with 100% ethanol.
  • Successful isolation of (E)-resveratrol (0.9 mg/g dry weight) and (E)-ε-viniferin (1.1 mg/g dry weight) with high purity.
  • Developed UHPLC-UV/DAD method validated for quantification.
  • Satisfactory results for greenness and environmental impact assessment.

Conclusions

  • Grapevine pruning waste is a viable and sustainable source of (E)-resveratrol and (E)-ε-viniferin.
  • The optimized MASE and chromatography procedure offers an eco-friendly approach for phytochemical extraction.
  • This method supports circular economy principles in viticulture by valorizing waste materials.

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