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Updated: Sep 18, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

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Mechanisms underlying hepatocellular carcinoma progression through N6-methyladenosine modifications of long

Ning Wang1, Fei-Tian Min1, Wei-Bo Wen1

  • 1The First School of Clinical Medicine, Yunnan University of Chinese Medicine, Kunming 650000, Yunnan Province, China.

World Journal of Gastroenterology
|June 20, 2025
PubMed

Insights

Long non-coding RNA KIF9-AS1 promotes cancer stemness and sorafenib resistance in hepatocellular carcinoma (HCC). This oncogenic role involves N6-methyladenosine modification, stabilizing SOX2, and offers a potential therapeutic target for HCC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Hepatocellular carcinoma (HCC) presents limited therapeutic options, especially in advanced stages.
  • Sorafenib resistance is a major challenge in HCC treatment.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer progression.

Discussion:

  • This study investigates the role of N6-methyladenosine-modified lncRNA KIF9-AS1 in HCC.
  • KIF9-AS1 functions as an oncogene by enhancing cancer stemness and sorafenib resistance.
  • Key regulatory proteins, including methyltransferase-like 3 and insulin-like growth factor 2 mRNA-binding protein 1, are involved in KIF9-AS1 modification.

Key Insights:

  • KIF9-AS1 stabilizes and upregulates SOX2 (short stature homeobox 2) via deubiquitination mediated by USP1 (ubiquitin-specific peptidase 1).
  • This stabilization of SOX2 enhances stemness properties in HCC cells.
  • The KIF9-AS1/USP1/SOX2 axis contributes significantly to sorafenib resistance in HCC.

Outlook:

  • KIF9-AS1 demonstrates potential as a diagnostic biomarker for HCC.
  • Targeting KIF9-AS1 presents a promising therapeutic strategy for overcoming sorafenib resistance.
  • Further clinical investigations are warranted to explore the therapeutic application of KIF9-AS1 in HCC management.
Keywords:
Hepatocellular carcinomaLong non-coding RNA KIF9-AS1N6-methyladenosineShort stature homeobox 2Sorafenib resistanceStemness

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