Related Experiment Video
Updated: Sep 18, 2025

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
Characterization of volatile compounds and physicochemical properties of Shiitake Mushrooms pickled using
Ashfaque Ahmed Khaskheli1,2, Shahzor Gul Khaskheli2, Wen Huang1
1College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070 Hubei China.
Abstract:
The present study was carried out to evaluate changes in the volatile composition and chemical characteristics of shitake mushroom pickled. The volatile components were characterized by methods based on headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The results showed that one sulfur compounds, 1,2,4-trithiolane and lenthionine, were identified in control. However, pickle inoculated with Lb. casei produced lenthionine compound was detected. The pickled inoculated with lactic culture Lb.BB-46 confirmed four sulfur compounds, 1,2,4-trithiolane, 1,2,4,5-tetrathiane, lenthionine and pentathiepane. Study further revealed that three organic acids were found in the Lb. casei sample, including malic acid, citric acid and acetic acid. The amount of flavor 5'- nucleotides in pickled mushroom inoculated with Lb.BB-46 was higher than that of Lb. casei and control sample. The change in pickled was most likely due to variations in quality, processing technique, and storage conditions of the pickled vegetable. We explained SPME/GC-MS as a practical instrument for monitoring volatile compounds in pickled and provide important information for investigating the process in fermentation products.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13197-024-06106-w.
More Related Videos
08:43PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
Published on: May 11, 2017
08:54Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry SESI-MS
Published on: June 8, 2011