Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

13.9K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.9K
Stringent Response in E. coli01:23

Stringent Response in E. coli

61
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
61
Factors Influencing Microbial Growth: Temperature01:27

Factors Influencing Microbial Growth: Temperature

253
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
253

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genomics accelerated isolation of a new stem rust avirulence gene-wheat resistance gene pair.

Nature plants·2021
Same author

Coking-resistant dry reforming of methane over Ni/γ-Al<sub>2</sub>O<sub>3</sub> catalysts by rationally steering metal-support interaction.

iScience·2021
Same author

Molecular identification and genetic-polymorphism analysis of Fasciola flukes in Dali Prefecture, Yunnan Province, China.

Parasitology international·2021
Same author

Resistance Mechanism to Metsulfuron-Methyl in <i>Polypogon fugax</i>.

Plants (Basel, Switzerland)·2021
Same author

CMMCSegNet: Cross-Modality Multicascade Indirect LGE Segmentation on Multimodal Cardiac MR.

Computational and mathematical methods in medicine·2021
Same author

Improved NO<sub></sub> Reduction over Phosphate-Modified Fe<sub>2</sub>O<sub>3</sub>/TiO<sub>2</sub> Catalysts <i>Via</i> Tailoring Reaction Paths by <i>In Situ</i> Creating Alkali-Poisoning Sites.

Environmental science & technology·2021

Related Experiment Video

Updated: Sep 18, 2025

Novel Assay for Cold Nociception in Drosophila Larvae
06:52

Novel Assay for Cold Nociception in Drosophila Larvae

Published on: April 3, 2017

7.8K

The molecular response of Neoseiulus bicaudus to cold acclimation.

Siqiong Tang1, Kaiqin Mu1, Xinqi Liang1

  • 1College of Agriculture, Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, Xinjiang 832003, China.

Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
|June 20, 2025
PubMed
Summary
This summary is machine-generated.

Cold acclimation significantly enhances the survival of the predatory mite Neoseiulus bicaudus at low temperatures. This process involves regulating energy metabolism and cellular protection mechanisms, including key genes like NbHSP70 and NbHSP90.

Keywords:
Heat shock proteinLow temperatureMitesProteomicTranscriptome

More Related Videos

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
06:01

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Published on: July 3, 2020

9.0K
High-Throughput Assays of Critical Thermal Limits in Insects
06:58

High-Throughput Assays of Critical Thermal Limits in Insects

Published on: June 15, 2020

5.3K

Related Experiment Videos

Last Updated: Sep 18, 2025

Novel Assay for Cold Nociception in Drosophila Larvae
06:52

Novel Assay for Cold Nociception in Drosophila Larvae

Published on: April 3, 2017

7.8K
Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
06:01

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans

Published on: July 3, 2020

9.0K
High-Throughput Assays of Critical Thermal Limits in Insects
06:58

High-Throughput Assays of Critical Thermal Limits in Insects

Published on: June 15, 2020

5.3K

Area of Science:

  • * Zoology
  • * Molecular Biology
  • * Environmental Science

Background:

  • * Neoseiulus bicaudus is a predatory mite vital for controlling spider mites.
  • * Temperature significantly impacts N. bicaudus distribution, growth, and development.
  • * Cold acclimation is a critical arthropod survival strategy against low temperatures.

Purpose of the Study:

  • * To investigate the effect of cold acclimation on N. bicaudus cold tolerance.
  • * To elucidate the molecular mechanisms underlying cold acclimation in N. bicaudus using multi-omics.
  • * To identify key genes and proteins involved in cold adaptation.

Main Methods:

  • * Transcriptome and proteomic analyses were performed on N. bicaudus subjected to varying cold acclimation regimes (3°C for 6h, 3°C for 24h, and 9°C for 7 days).
  • * Survival rates at acute low temperatures (-6°C) were assessed.
  • * RNA interference (RNAi) was used to evaluate the function of specific genes (NbHSP70, NbHSP90).

Main Results:

  • * Cold acclimation, particularly the 7-day treatment, significantly increased N. bicaudus survival time at -6°C.
  • * Multi-omics data revealed coordinated regulation of energy metabolism (suppression of synthesis, enhancement of oxidation and phosphorylation) and cellular protection pathways.
  • * Upregulation of genes/proteins involved in mRNA processing, translation, protein folding, and degradation was observed, supporting homeostasis.
  • * RNAi experiments confirmed the crucial role of NbHSP70 and NbHSP90 in cold tolerance.

Conclusions:

  • * Cold acclimation enhances the cold tolerance of Neoseiulus bicaudus through sophisticated molecular adjustments.
  • * Key molecular mechanisms involve metabolic reprogramming for energy conservation and enhanced cellular protection and repair systems.
  • * NbHSP70 and NbHSP90 are critical regulators of cold tolerance in this beneficial mite species.