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Regulation of AMPs expression by six Tolls in stomach during WSSV infection in Macrobrachium nipponense

Qian Ren1, Ying Huang2, Chen Qu3

  • 1State Key Laboratory of Climate System Prediction and Risk Management, Nanjing University of Information Science and Technology, Nanjing, 210044, China; School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, 210044, China.

Fish & Shellfish Immunology
|June 20, 2025
PubMed

Insights

Six Toll genes in the oriental river prawn Macrobrachium nipponense are crucial for innate immunity. These Toll-like receptors (TLRs) regulate antimicrobial peptides, enhancing survival against white spot syndrome virus (WSSV) infection.

Area of Science:

  • * Invertebrate immunology
  • * Molecular biology
  • * Crustacean aquaculture

Background:

  • * Toll-like receptors (TLRs) are key components of innate immunity in diverse organisms.
  • * Understanding TLRs in crustaceans like Macrobrachium nipponense is vital for disease resistance.

Purpose of the Study:

  • * To identify and characterize Toll genes in Macrobrachium nipponense.
  • * To investigate the role of these Toll genes in the prawn's immune response to white spot syndrome virus (WSSV).

Main Methods:

  • * Identification and classification of six Toll genes (MnToll1-6) based on domain structure and evolutionary analysis.
  • * Analysis of MnToll gene expression in various tissues and upon WSSV challenge using quantitative PCR.
  • * Functional analysis through RNA interference (RNAi) to assess the impact of MnToll knockdown on WSSV infection and survival.

Main Results:

  • * Six Toll genes (MnToll1-6) were identified, with varying domain structures and evolutionary clustering.
  • * MnToll genes were widely distributed and highly expressed in the stomach, with upregulation upon WSSV infection.
  • * Knockdown of MnToll genes led to decreased expression of antimicrobial peptides, increased WSSV loads, and reduced prawn survival.

Conclusions:

  • * Macrobrachium nipponense possesses a diverse set of Toll genes involved in innate immunity.
  • * These Toll genes play a significant role in defense against WSSV by modulating antimicrobial peptide production.
  • * The findings highlight the importance of Tolls in prawn immune defense and offer insights for aquaculture disease management.
Keywords:
ALFsEvolutionInnate immuneTollsWSSV

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