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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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Split technology in sensors based on CRISPR/Cas12a system.

Xianmin Ding1, Xueying Lei2, Songcheng Yu2

  • 1Department of Nuclear Medicine, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou 450008, China.

Biotechnology Advances
|June 20, 2025
PubMed
Summary
This summary is machine-generated.

Split technology enhances the CRISPR/Cas12a system for nucleic acid analysis, enabling detection of new targets like RNA and expanding programmability for advanced biosensors.

Keywords:
CRISPRCas12aSensorSplit

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Biosensor Technology

Background:

  • The CRISPR/Cas12a system is a powerful tool for nucleic acid analysis, known for its specificity, sensitivity, and programmability.
  • Recent advancements involve applying split technology to various components of the CRISPR/Cas12a system, including activators, crRNA, reporters, and Cas12a.

Purpose of the Study:

  • To review the advancements of split technology in CRISPR/Cas12a-based sensors.
  • To summarize the benefits of split technology for CRISPR/Cas12a systems.
  • To discuss challenges and future perspectives for these sensors.

Main Methods:

  • Review of literature on split technology applied to CRISPR/Cas12a systems.
  • Analysis of how split components enable detection of novel targets.
  • Evaluation of the impact on sensor cost, stability, and programmability.

Main Results:

  • Split technology expands the detection capabilities of CRISPR/Cas12a systems to include dsDNA without PAM, short ssDNA (<15 nt), and RNA.
  • It allows for the development of label-free, lower-cost sensors.
  • Split technology facilitates the construction of cellular logic circuits with multiple inputs and outputs.

Conclusions:

  • Split technology significantly enhances the CRISPR/Cas12a system by expanding target scope, improving crRNA stability, increasing strategic programmability, and reducing detection costs.
  • These advancements pave the way for more versatile and cost-effective nucleic acid detection platforms.