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Published on: May 11, 2020
Haplotype-resolved genome assembly of the tetraploid potato cultivar Désirée
Tim Godec1,2, Sebastian Beier3, Natalia Yaneth Rodriguez-Granados4
1National Institute of Biology, Department of Biotechnology and Systems Biology, Ljubljana, Slovenia. tim.godec@nib.si.
Abstract:
Cultivar Désirée is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Désirée, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in a high-contiguity chromosome-level assembly. We implemented a comprehensive annotation pipeline incorporating gene models and functional annotations from the Solanum tuberosum Phureja DM reference genome alongside RNA-seq reads to provide high-quality gene and transcript annotations. Additionally, we provide a genome-wide DNA methylation profile using Oxford Nanopore reads, enabling insights into potato epigenetics. The assembled genome, annotations, methylation and expression data are visualised in a publicly accessible genome browser, providing a valuable resource for the potato research community.

