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Dual-Cross-linked Methylacrylated Collagen-DPPA Bioinks for Precision DLP Bioprinting and Accelerated Skin Wound
Lang Xiao1,2, Mingzhu Ye1,2, Yirui Fan1,2
1State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.
Biomacromolecules
|June 21, 2025
Summary
A new dual-network collagen bioink, methyl acrylated collagen-dimethylphenylphosphonate (CMA-DPPA), enhances digital light processing (DLP) 3D bioprinting. This innovative bioink creates robust, printable cell-laden constructs for tissue engineering and wound healing.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Digital Light Processing (DLP) bioprinting offers speed and precision for tissue engineering.
- Developing bioinks with improved printability, bioactivity, and cell support is crucial.
- Current bioinks face limitations in mechanical strength and print fidelity.
Purpose of the Study:
- To introduce a novel dual-network collagen-based bioink for DLP 3D bioprinting.
- To enhance the mechanical robustness, printability, and bioactivity of bioprinted constructs.
- To evaluate the efficacy of the new bioink in a preclinical wound healing model.
Main Methods:
- Synthesis of a dual-network hydrogel: methyl acrylated collagen-dimethylphenylphosphonate (CMA-DPPA).
- Fabrication of cell-laden constructs using DLP 3D bioprinting.
- Assessment of hydrogel properties: printability, mechanical strength, biocompatibility, and degradation.
- In vivo evaluation in a rat model of full-thickness skin defects.
Main Results:
- The CMA-DPPA bioink demonstrated high printing fidelity and mechanical robustness.
- Dual-crosslinking significantly improved structural integrity and resistance to enzymatic degradation.
- The hydrogel supported cell adhesion, proliferation, and migration.
- In vivo studies showed accelerated wound closure and organized tissue regeneration.
Conclusions:
- The CMA-DPPA bioink is a promising material for DLP 3D bioprinting.
- It enables the fabrication of mechanically stable and bioactive scaffolds.
- This technology holds potential for advancing tissue engineering and regenerative medicine applications, particularly in wound healing.
