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Updated: Sep 18, 2025

Fiber Type and Subcellular-Specific Analysis of Lipid Droplet Content in Skeletal Muscle
Published on: June 8, 2022
Characteristics of ergothioneine distribution across skeletal muscles and adipose tissues
Kentaro Nishioka1, Takahiro Ishimoto1, Makoto Katsube1
1Faculty of Pharmacy, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
Abstract:
Ergothioneine (EGT), a food-derived amino acid, is distributed to various organs via the carnitine/organic cation transporter, OCTN1. However, the distribution characteristics of EGT across different skeletal muscles and adipose tissues remains unexplored. This study evaluated the distribution of EGT and OCTN1 mRNA expression in the soleus, gastrocnemius, plantaris muscle, epididymal white adipose tissue (eWAT), and interscapular brown adipose tissue (iBAT) of middle-aged C57BL/6 J wild-type male mice, which were orally administered 20 mg/kg of EGT once daily for up to 56 days. EGT was distributed at significantly higher concentrations in the soleus than in the gastrocnemius and plantaris muscles. Similarly, the mRNA level of OCTN1 was significantly higher in the soleus muscle. Regarding adipose tissue, EGT was distributed at significantly higher concentrations in iBAT than in eWAT. The soleus and iBAT may have a higher risk of oxidative stress, which could be a probable reason for the higher distribution of the antioxidant EGT. This study revealed the unique characteristics of EGT distribution in skeletal muscles and adipose tissues, providing a solid foundation for future studies on EGT health strategies.
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