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Updated: Sep 8, 2025
07:09
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
Published on: May 28, 2021
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Tools and databases for non-coding RNAs
Akshaykumar Zawar1, Srinka Datta2, Rishita Rathi2
1GeneSpectrum Life Sciences LLP, Pune, Maharashtra, India; Department of Life Sciences, School of Science and Mathematics, DES Pune University, Pune, Maharashtra, India.
Progress in Molecular Biology and Translational Science
|June 21, 2025
Summary
Non-coding RNAs (ncRNAs) are vital genetic elements, not "junk DNA," influencing cellular processes and gene regulation. Research highlights ncRNA roles in health and disease, with new methods for detection and analysis.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- Non-coding RNA (ncRNA) regions were once considered genomic "junk DNA."
- Advances in sequencing reveal ncRNAs are crucial for cellular processes and gene regulation.
- The role of ncRNAs in human health and disease is increasingly recognized.
Purpose of the Study:
- To explore the current landscape of ncRNA research.
- To highlight techniques for detecting and identifying ncRNA classes.
- To discuss computational tools for ncRNA annotation and functional analysis.
Main Methods:
- Review of molecular biology and sequencing technologies.
- Discussion of experimental and computational approaches.
- Analysis of ncRNA identification, annotation, and classification methods.
Main Results:
- ncRNAs are essential genetic elements influencing gene regulation.
- Genetic variations in ncRNA genes are linked to disease development.
- Integrated approaches enhance understanding of ncRNA functions.
Conclusions:
- ncRNA research is rapidly evolving, moving beyond the "junk DNA" concept.
- Understanding ncRNA variations offers insights into gene regulation and disease.
- Further research integrating experimental and computational methods can unlock therapeutic applications.
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