Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K
Types of RNA01:20

Types of RNA

6.3K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
6.3K
RNA Interference01:23

RNA Interference

26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.4K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.4K
MicroRNAs01:22

MicroRNAs

21.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.6K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In silico approaches to improved understanding of herbicides targeting photosystem II (PSII).

Computational biology and chemistry·2026
Same author

Toward the genetic landscape of prostate cancer in India: insights from whole-exome and low-pass whole-genome sequencing of formalin-fixed paraffin-embedded tumor tissues.

Frontiers in systems biology·2026
Same author

Integrating multi-omics data for next-generation cancer research and precision medicine.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2026
Same author

Draft genome sequencing of Proteus mirabilis strain Indica (ST286) reveals antimicrobial resistance and virulence determinants.

BMC microbiology·2026
Same author

Distinct genetic variants from the whole-exome sequencing of syndromic anorectal malformations: A cross-sectional study.

iScience·2026
Same author

Isolation and genomic characterization of a lytic bacteriophage against multidrug-resistant Proteus mirabilis.

Applied microbiology and biotechnology·2026
Same journal

Artificial intelligence-driven multi-omics analysis of gut-kidney axis in chronic kidney disease.

Progress in molecular biology and translational science·2026
Same journal

Artificial intelligence in multi-omics analysis of heart diseases.

Progress in molecular biology and translational science·2026
Same journal

AI in multi-omics analysis of type 2 diabetes.

Progress in molecular biology and translational science·2026
Same journal

AI in multi-omics analysis in AMR.

Progress in molecular biology and translational science·2026
Same journal

AI in multi-omics analysis of COVID-19 patient data.

Progress in molecular biology and translational science·2026
Same journal

AI in multi-omics analysis of liver diseases.

Progress in molecular biology and translational science·2026
See all related articles

Related Experiment Video

Updated: Sep 8, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.6K

Non-coding RNAs and their integrated biological networks.

Bilal Ahmed Abbasi1, Renuka Suravajhala2, V S Sundararajan2

  • 1TCS Genomics Lab, Tata Consultancy Services Limited, Noida, India; Bioclues.org, Hayatnagar, Hyderabad, India.

Progress in Molecular Biology and Translational Science
|June 21, 2025
PubMed
Summary
This summary is machine-generated.

Non-coding RNAs (ncRNAs) are crucial gene regulators, influencing cellular functions and diseases like cancer. This chapter explores their roles, interactions with coding genes, and analysis tools, highlighting clinical significance.

Keywords:
Functional genomicsGene regulationNcRNANcRNA annotationin-silico tools

More Related Videos

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.8K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.2K

Related Experiment Videos

Last Updated: Sep 8, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.6K
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.8K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Non-coding RNAs (ncRNAs) were previously overlooked as transcriptional noise but are now recognized as critical regulators.
  • ncRNAs play vital roles in gene expression, cellular function, epigenetic modifications, and RNA stability.
  • Their functions extend beyond previously perceived passive roles in cellular processes.

Purpose of the Study:

  • To explore the diverse regulatory functions of ncRNAs.
  • To examine the intricate interplay between coding and non-coding gene networks.
  • To review computational tools for ncRNA identification, annotation, and analysis.

Main Methods:

  • Literature review of ncRNA functions and regulatory mechanisms.
  • Analysis of existing studies on ncRNA involvement in disease pathophysiology.
  • Compilation of available in silico tools and methodologies for ncRNA research.

Main Results:

  • ncRNAs act as transcriptional modulators by binding to DNA/proteins or interacting with transcription factors.
  • ncRNAs are implicated in the pathophysiology of diseases, notably cancer and neurological disorders.
  • A range of in silico tools are available for the comprehensive analysis of ncRNAs.

Conclusions:

  • ncRNAs are essential regulators of gene expression with significant functional and clinical implications.
  • Understanding ncRNA networks is crucial for deciphering cellular functions and disease mechanisms.
  • Further research into ncRNAs and their associated tools will advance diagnostics and therapeutics for various diseases.