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Updated: May 12, 2026
Experimental Protocol to Investigate Particle Aerosolization of a Product Under Abrasion and Under Environmental Weathering
Published on: September 16, 2016
Profilometric and scanning electron microscopy analysis comparing hydroxyapatite and zinc oxide nanoparticles for
Mayar H Hassaan1, Nagah A Rashad2, Afaf A El Sawa2
1Department of Oral Biology, College of Dentistry, Arab Academy for Science, Technology & Maritime Transport, El Alamein, Egypt. mayarhamdy@aast.edu.
Background:
Dentin hypersensitivity (DH) is a prevalent dental issue characterized by sharp pain of short duration. Although the exact cause of DH remains debated, the hydrodynamic theory explains its mechanism. Furthermore, various methods, such as potassium-containing toothpaste, mouthwash, and chewing gum, have been attempted to address DH. However, recent research has explored the potential of nanoparticles (NPs) for DH treatment due to their biocompatibility and bioactive properties.
Methods:
Twenty-seven sound premolars extracted for orthodontic purposes were utilized in the present study to obtain 27 mid-coronal dentin discs. These discs were then etched with 37% orthophosphoric acid for 20 s to expose dentinal tubules (DTs) and simulate DH. Subsequently, dentin discs were rinsed with distilled water for 1 min. These dentin discs were randomly allocated into 3 groups: Group I (etched control), Group II (the etched dentin discs were treated with a carboxymethyl cellulose (CMC) dental hydrogel scaffold loaded with 20% HANPs), and Group III (the etched dentin discs were treated with a CMC dental hydrogel scaffold loaded with 20% ZnO NPs). After 7 days, Group II and III underwent erosive challenge to evaluate the protective effect of the nanoparticle treatments. Finally, the dentin discs were analyzed using profilometric analysis to measure surface roughness (SRa), scanning electron microscopy (SEM) to evaluate DT occlusion, computer-assisted SEM image analysis using Image J.
Results:
Among the groups, Group I exhibited the highest SRa following the DH simulation. Group II showed lower SRa compared to group III. SEM analysis indicated that Group III displayed more occluded DTs compared to Group II. Group I showed the most unoccluded DTs. The SEM analysis results were further quantitatively confirmed using Image J.
Conclusions:
The results indicated that both HANPs and ZnO NPs exhibited promising outcomes; however, ZnO NPs demonstrated superior effectiveness in resisting erosive wear compared to HANPs.
