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Updated: Sep 18, 2025

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
Published on: May 24, 2024
Evaluation of the I-dOne system for rapid bacterial identification: A diagnostic accuracy study
Felipe Francisco Tuon1, Paula Hansen Suss1, Gabriel Burato Orthis1
1Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, PR 80215-901, Brazil.
None:
This study assessed the I-dOne® system (ATR-FTIR) for bacterial identification versus MALDI-TOF MS using 436 clinical isolates. Overall concordance was 90.2 %, with high accuracy for Gram-positive cocci and major Gram-negatives. Despite lower performance for some Enterobacterales, i-dOne offers a rapid, affordable diagnostic alternative where MALDI-TOF is unavailable.

