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The Utility of Fourier Transform Infrared Spectroscopy (FTIR) for Detecting Exercise-Induced Changes in the Human
Paweł Król1, Zbigniew Obmiński2, Adam Reich3
1Department of Physical Education, Medical College of Rzeszow University, Rzeszow, Poland.
Journal of Biophotonics
|June 22, 2025
Summary
Physical exercise causes transient changes in skin's infrared spectra. Fourier Transform Infrared Spectroscopy (FTIR) detected significant shifts in epidermal lipids, phospholipids, and carbohydrates after moderate exercise in athletes.
Area of Science:
- Biomedical Engineering
- Dermatology
- Spectroscopy
Background:
- Limited data exists on how physical activity impacts epidermal biochemical composition.
- Understanding these changes is crucial for sports science and dermatology.
Purpose of the Study:
- To investigate transient infrared spectral alterations in the epidermis post-exercise.
- To test if moderate exercise affects specific spectral bands in healthy individuals.
Main Methods:
- Fourier Transform Infrared Spectroscopy (FTIR) analyzed epidermal samples from athletes.
- Samples were collected pre- and post- a 1.5-hour moderate-intensity training session.
- Absorbance values of 12 spectral peaks were quantified.
Main Results:
- Significant correlations were found for peaks at 3270 cm⁻¹ and 1045 cm⁻¹.
- Post-exercise increases in absorbance were observed for lipids/proteins (1453 cm⁻¹), phospholipids (1078 cm⁻¹), and carbohydrates (1045 cm⁻¹).
- High inter-individual variability was noted, with a general upward trend in other peaks.
Conclusions:
- Fourier Transform Infrared Spectroscopy (FTIR) can detect acute biochemical responses in the epidermis following exercise.
- Exercise-induced changes are particularly evident in lipid and phospholipid structures.
- Further research is needed to explore these transient spectral modifications.
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