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Updated: Sep 18, 2025

Using Near-Infrared Spectroscopy Wearable Devices to Identify Central Versus Peripheral Limitations During Exercise
Published on: December 19, 2024
The Utility of Fourier Transform Infrared Spectroscopy (FTIR) for Detecting Exercise-Induced Changes in the Human
Paweł Król1, Zbigniew Obmiński2, Adam Reich3
1Department of Physical Education, Medical College of Rzeszow University, Rzeszow, Poland.
Abstract:
The literature lacks data on transient infrared spectral changes in the epidermis following physical exercise. This study tested the hypothesis that a single exercise session affects selected spectral bands (3270-1045 cm-1) in healthy individuals. Eight professional tennis players completed a 1.5-h moderate-intensity training session. Epidermal samples from the inner hand were collected before and after exercise, following cleaning with distilled water and 96% PA ethyl alcohol. Samples were analyzed using Fourier Transform Infrared Spectroscopy (FTIR). Absorbance values were recorded for 12 peaks. Significant correlations were observed for the 3270 cm-1 (r = 0.976) and 1045 cm-1 (r = 0.754) peaks. Notably, post-exercise increases were found at 1453 cm-1 (lipids/proteins), 1078 cm-1 (phospholipids), and 1045 cm-1 (carbohydrates). No significant changes were observed for other peaks, though a general upward trend appeared. Inter-individual variability was high. FTIR may detect acute epidermal biochemical responses to exercise, especially in lipid- and phospholipid-related structures.
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