Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

9.0K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
9.0K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.4K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.4K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K
Gastrulation01:56

Gastrulation

58.8K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
58.8K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polystyrene Nanoplastics Disrupt Mouse Placenta Development in a Sex-Dependent Manner.

bioRxiv : the preprint server for biology·2026
Same author

Programmable morphogenesis: integrating biophysical and genetic engineering tools to direct tissue formation.

Biofabrication·2026
Same author

YAP-driven malignant reprogramming of oral epithelial stem cells at single cell resolution.

Nature communications·2025
Same author

Vascularized liver-on-a-chip model to investigate nicotine-induced dysfunction.

Biomicrofluidics·2023
Same author

'Chip'-ing away at morphogenesis - application of organ-on-chip technologies to study tissue morphogenesis.

Journal of cell science·2023
Same author

YAP-Driven Oral Epithelial Stem Cell Malignant Reprogramming at Single Cell Resolution.

bioRxiv : the preprint server for biology·2023
Same journal

A Programmed Drug-Loaded and Penetration-Delivery Functionalized Microneedle Patch for Synergistic Obesity Treatment.

Advanced healthcare materials·2026
Same journal

Antibacterial and Immunomodulatory Coatings for Orthopedic Metal Implants: Biological Rationale, Design Strategies, and Translational Challenges.

Advanced healthcare materials·2026
Same journal

Butyrylated PGAM5-Triggered and GSH-Responsive Cysteine Polymer Nanoparticles for CBL0137 Delivery to Enhance Necroptosis in Prostate Cancer.

Advanced healthcare materials·2026
Same journal

Dual-Modal Phototherapeutic Nanoagents Eradicating Drug-Resistant Bacteria via Multi-Pathway of Membrane Disruption, Oxidative Damage, and Energy Metabolism Interference.

Advanced healthcare materials·2026
Same journal

Smartphone-Enabled Point-of-Care Biosensing Platform With Self-Calibration for Rapid Matrix-Resistant Detection of Multiple AMI Biomarkers in Whole Blood.

Advanced healthcare materials·2026
Same journal

Multimetal-Doped Nanoenzymes Reprogram Macrophages for Immunotherapy of Gouty Arthritis.

Advanced healthcare materials·2026
See all related articles

Related Experiment Video

Updated: Sep 18, 2025

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.5K

Geometrically Controlled WNT Activation Drives Intestinal Morphogenesis.

Nathaniel C Burmas1, Arlyn M Fabian2, Amoli Vanavadiya1

  • 1Department of Chemical and Biomolecular Engineering, Irvine, CA, 92697, USA.

Advanced Healthcare Materials
|June 23, 2025
PubMed
Summary
This summary is machine-generated.

Engineered microenvironments and WNT signaling modulation promote intestinal epithelial morphogenesis. Mechanical and architectural cues independently drive tissue formation, offering new gut-mimetic culture strategies.

Keywords:
bioprintingintestinemechanotransductionmorphogenesistissue engineering

More Related Videos

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.4K
The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

8.1K

Related Experiment Videos

Last Updated: Sep 18, 2025

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.5K
Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.4K
The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

8.1K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • Morphogenesis integrates biochemical, mechanical, and architectural cues for tissue formation.
  • Colorectal carcinoma (Caco-2) cells serve as a model for studying intestinal epithelial development.

Purpose of the Study:

  • To investigate the synergistic effects of WNT signaling modulation and engineered microenvironments on crypt-villus-like morphogenesis in Caco-2 cells.
  • To elucidate the independent roles of mechanical and architectural factors in orchestrating intestinal epithelial morphogenesis.

Main Methods:

  • Utilized fibroblast conditioned media and WNT agonist (CHIR) to modulate WNT signaling.
  • Employed digital light processing (DLP) printing to fabricate gelatin methacrylate (GelMA) microwell arrays for controlled microenvironments.
  • Assessed epithelial budding and mucin 2 (MUC2) expression.
  • Disrupted myosin contractility to evaluate mechanotransduction.

Main Results:

  • Fibroblast conditioned media induced WNT-dependent epithelial budding, enhanced by CHIR.
  • Engineered GelMA microwells with increased scaffold perimeter-to-area ratios promoted budding and MUC2 upregulation.
  • Mechanical cues, specifically myosin contractility, were crucial for these morphogenetic effects.

Conclusions:

  • WNT signaling and engineered microenvironments synergistically drive crypt-villus-like morphogenesis.
  • Mechanical and architectural cues can independently orchestrate intestinal epithelial morphogenesis.
  • Findings offer novel strategies for developing gut-mimetic culture systems.