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Improving resolution with single-cell detection of CRISPR off-target events

Ciaran M Lee1

  • 1School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.

Molecular Therapy. Methods & Clinical Development
|June 23, 2025
PubMed
Summary

No abstract available in PubMed .

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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