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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
Published on: April 11, 2013
Alternarias G and H: Benzoxepine Derivatives from the Endophytic Fungus Alternaria sp. HJT-Y7
Dongliang Xiao1,2,3, Shuangcheng Li1, Xiaoyuan Tang1,4
1College of Life and Health, Dalian University, Dalian 116622, People's Republic of China.
Abstract:
Alternaria G (1), a benzoxepine dimer with an unprecedented 6/7-7/6 tetracyclic skeleton, and a benzoxepine monomer, alternaria H (2), were successfully isolated from the fungus Alternaria sp. HJT-Y7. Structural determination was accomplished through comprehensive spectroscopic data interpretation, supported by electronic circular dichroism calculations, and quantum-chemical NMR calculations with DP4+ probability analyses. Genomic exploration uncovered a candidate biosynthetic gene cluster (aln) putatively responsible for their assembly, from which a plausible biosynthetic route was proposed. Notably, 1 significantly inhibited nitric oxide production in LPS-stimulated RAW 264.7 macrophages.
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