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Related Experiment Video

Updated: Sep 18, 2025

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Published on: August 30, 2016

Assessing Campylobacter jejuni Extracellular Vesicle-Host Interaction Using a Microfluidic Platform with Caco-2

Silvia Tea Calzuola^1,2, Jeanne Malet-Villemagne³, Debora Pinamonti⁴

¹UMR7646 Laboratoire d'hydrodynamique (LadHyX), Ecole Polytechnique, Palaiseau 91128, France.

ACS Biomaterials Science & Engineering

|June 23, 2025

Summary

Campylobacter jejuni extracellular vesicles (EVs) are less harmful to 3D intestinal cell cultures than 2D cultures. A new microfluidic device monitors these interactions, revealing protective 3D cell organization against pathogen EVs.

Keywords:

bacterial extracellular vesicles bioengineering campylobacter gut epithelium model host-bacterial interaction spheroides

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Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

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Last Updated: Sep 18, 2025

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Published on: August 30, 2016

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Published on: April 20, 2010

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Area of Science:

Microbiology
Biomedical Engineering
Cell Biology

Background:

Campylobacter jejuni is a foodborne pathogen.
Extracellular vesicles (EVs) from C. jejuni contribute to pathogenicity.
The role of C. jejuni EVs in host cell invasion is not well understood.
Existing in vitro and animal models have limitations in studying host-pathogen interactions.

Purpose of the Study:

To develop and validate a microfluidic platform for studying C. jejuni EV interactions with human intestinal epithelial cells.
To investigate the impact of 3D cell culture models on host cell response to C. jejuni EVs.
To monitor real-time host-microbe interactions using an integrated impedimetric sensor.

Main Methods:

Development of a microfluidic device for culturing Caco-2 cells.
Induction of 3D Caco-2 cell spheroid formation within the microfluidic device.
Utilizing impedance spectroscopy and live microscopy for real-time monitoring.
Assessing Caco-2 cell viability using MTT assays.
Measuring transepithelial electrical resistance (TEER) to evaluate barrier integrity.

Main Results:

Caco-2 cells spontaneously formed 3D spheroids in the microfluidic device.
3D Caco-2 spheroids exhibited enhanced resistance to C. jejuni secretome and EVs compared to 2D cultures.
The microfluidic platform enabled real-time detection of C. jejuni EV interactions with intestinal cells.
TEER measurements indicated paracellular diffusion of C. jejuni EVs.
3D cell organization demonstrated a protective role against C. jejuni EV cytotoxicity.

Conclusions:

The microfluidic impedimetric platform is effective for studying host-pathogen interactions.
3D intestinal cell models provide a more physiologically relevant system for evaluating pathogen virulence.
C. jejuni EVs exhibit different interactions and effects in 3D versus 2D cell cultures.
The developed platform offers a promising tool for gastroenteritis research and understanding host-microbe dynamics.