Study on the chemical composition, zebrafish toxicity and anti-inflammatory activity of Ecklonia kurome

Inflammation is an important physiological process that serves as the host's defense mechanism against tissue damage, stress, or oxidative stress. As a traditional medicinal and edible plant, Ecklonia kurome (EK) is used to treat galls and scrofula, which is now known as lymphatic tuberculosis and lymphadenitis, but the specific effective medicinal ingredients of EK are not clear, and further exploration is needed for its anti-inflammatory activity.

This study aims to extract, isolate and purify EK, and investigate its pharmacological effects, in order to explore the chemical composition, toxicity and anti-inflammatory activity of EK.

Silica gel, Sephadex gel (LH-20) and other column chromatography methods were used to separate and purify the ethanol extract of EK, and the zebrafish embryo model was used to conduct toxicity evaluation research. The anti-inflammatory activity of monomer compounds was predicted and verified based on molecular docking technology. The lipopolysaccharide induced cell inflammation model was tested to detect NO, reactive oxygen species (ROS), interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) Study the expression level of indicators and investigate the therapeutic effect of EK on inflammatory cells.

Eight compounds were isolated from EK, namely Ishigoside (1), Squalene (2), δ-tocopherol (3), Phytol (4), Di - (2-ethylhexyl) phthalate (5), 1,3-dilinoloylglycerol (6), Fuchosterol (7), and Mannitol (8). Compound 1 was isolated from EK for the first time, while compounds 2-6 were reported for the first time.; The toxicity test results showed that compounds 1-8 did not produce zebrafish embryonic developmental toxicity at 12.5, 25, 50, and 100 μ M; The molecular docking results showed that the new compound had a good binding effect with inflammatory factors; The anti-inflammatory experiment results of RAW264.7 cells showed that compounds 1-8 did not produce cytotoxicity at 3, 10, 30, and 100 nM, but significantly increased cell viability and inhibited NO in cells, The levels of ROS, IL-6, IL-1β, TNF-α, and PGE2.

The research results indicate that compounds derived from EK can significantly reduce the expression levels of inflammatory cytokines and are safe, suggesting that EK has certain application prospects and can be further developed and utilized.