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Updated: Sep 18, 2025
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In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves
Published on: April 13, 2014
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Intrinsic activation of the coagulation pathway induces mouse sciatic nerve hypoexcitability
Shani Berkowitz1, Valery Golderman1, Zehavit Goldberg1
1Department of Neurology and Neurosurgery, Gray Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, 6997801, Israel; Department of Neurology, The Chaim Sheba Medical Center, Ramat Gan, 52626202, Israel.
Neuropharmacology
|June 23, 2025
Summary
The intrinsic FX-thrombin pathway in mouse sciatic nerves reduces neuronal excitability via PAR1. This highlights coagulation
Area of Science:
- Neuroscience
- Hematology
- Biochemistry
Background:
- Coagulation factors like thrombin and its receptor PAR1 influence neuronal function.
- The role of intrinsic neuronal coagulation factor activation in peripheral nerves is not well understood.
Purpose of the Study:
- To investigate the impact of intrinsic coagulation pathway activation on peripheral nerve function.
- To explore the role of the FX-thrombin-PAR1 axis in sciatic nerve excitability.
Main Methods:
- Utilized an ex vivo mouse sciatic nerve model.
- Administered Russell's viper venom factor X (FX) activator (RVV-X), FX inhibitor (apixaban), thrombin, or PAR1 antagonist (SCH 79797).
- Assessed neural excitability via electrophysiological recordings and quantified coagulation factors.
Main Results:
- RVV-X and thrombin significantly reduced neural responses to stimulation.
- Apixaban and the PAR1 antagonist blocked these inhibitory effects.
- Coagulation components were localized to the paranodal region of the sciatic nerve.
Conclusions:
- The FX-thrombin pathway is intrinsically active in mouse sciatic nerves.
- Pathway activation decreases neuronal excitability through PAR1, indicating a feedback mechanism.
- Coagulation pathways may be implicated in peripheral nerve diseases, offering potential therapeutic targets.
