Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pinocytosis00:38

Pinocytosis

3.4K
Cells use energy-requiring bulk transport mechanisms to transfer large particles or large numbers of small particles into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
Pinocytosis ("cellular drinking") is one of three main types of...
3.4K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

4.6K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.6K
Cell Size01:22

Cell Size

118.6K
Cell sizes vary widely among and within organisms. Bacterial cells range between 1-10 micrometers (μm)and are considerably smaller than most eukaryotic cells. The smallest bacteria are 0.1 μm in diameter—about a thousand times smaller than eukaryotic cells, which typically range from 10-100 μm.
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...
118.6K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.3K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crashing by design: Utilizing DNA damage for MCC differentiation.

Trends in cell biology·2026
Same author

Sidekick2 facilitates multiciliated cell penetration through multicellular adherens junctions.

bioRxiv : the preprint server for biology·2026
Same author

Travelling Waves in Gene Expression: A Mathematical Model of Cell-State Dynamics in Melanoma.

Bulletin of mathematical biology·2026
Same author

Identification of <i>Streptococcus pyogenes</i> isolates with reduced beta-lactam susceptibility in a cohort of children with pharyngitis.

Antimicrobial agents and chemotherapy·2026
Same author

Making the invisible visible: A global examination of careers and recognition for Imaging Scientists in core facilities.

Journal of microscopy·2026
Same author

Efficacy of Tonlamarsen in Patients With Uncontrolled Hypertension: The KARDINAL Phase 2 Randomized Clinical Trial.

Journal of the American College of Cardiology·2026

Related Experiment Video

Updated: Sep 18, 2025

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
08:05

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Published on: May 27, 2021

2.7K

Apical size reduction by macropinocytosis alleviates tissue crowding.

Enzo Bresteau1, Eve E Suva1, Christopher Revell2

  • 1Department of Cell and Developmental Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Nature Communications
|June 23, 2025
PubMed
Summary

Tissue crowding is managed by macropinocytosis, a process where cells shrink their apical surface to avoid extrusion. This mechanism aids in epithelial remodeling and regulates cell extrusion during development and compression.

More Related Videos

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
11:01

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Published on: August 24, 2021

2.9K
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
07:31

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

Published on: August 19, 2021

2.5K

Related Experiment Videos

Last Updated: Sep 18, 2025

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
08:05

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Published on: May 27, 2021

2.7K
Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
11:01

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes

Published on: August 24, 2021

2.9K
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
07:31

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

Published on: August 19, 2021

2.5K

Area of Science:

  • Cell biology
  • Developmental biology
  • Tissue engineering

Background:

  • Epithelial tissues face challenges from crowding, often leading to cell extrusion.
  • Live cell extrusion is a destructive process for tissue integrity.

Purpose of the Study:

  • To investigate the role of macropinocytosis in alleviating tissue crowding.
  • To understand how macropinocytosis regulates epithelial remodeling and cell extrusion.

Main Methods:

  • Studied mechanosensory signaling triggering macropinocytosis in response to crowding.
  • Observed macropinocytosis during epithelial development and in response to external compression.
  • Inhibited macropinocytosis to assess its effect on cell extrusion.

Main Results:

  • Macropinocytosis reduces apical surface area, alleviating tissue crowding without cell loss.
  • This mechanism is triggered by crowding via mechanosensory signaling.
  • Inhibiting macropinocytosis significantly increases cell extrusion, indicating a cooperative role.

Conclusions:

  • Macropinocytosis is a key mechanism for dynamic epithelial remodeling.
  • It acts as a less destructive alternative to cell extrusion.
  • Macropinocytosis regulates epithelial organization and the timing of cell extrusion.