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Updated: Sep 18, 2025
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Published on: November 11, 2016
Investigating the Relationship between Ulcerative Colitis and Circadian Rhythm Genes Using Bioinformatics Analysis
Jianghao Wang1, Meiyi You1, Jichang Li1
1Department of Gastrointestinal Surgery, Peking University First Hospital, Peking University, Beijing, 100034, People's Republic of China.
Circadian rhythm disruptions are linked to ulcerative colitis (UC). Key genes PPARGC1A and SLC6A4 show altered expression and correlate with immune cells, suggesting potential diagnostic biomarkers for UC.
Area of Science:
- Immunology
- Genetics
- Chronobiology
Background:
- Ulcerative colitis (UC) pathogenesis is complex, involving immune dysregulation.
- Circadian rhythm disruptions are increasingly implicated in inflammatory bowel diseases like UC.
Purpose of the Study:
- To investigate the correlation between circadian rhythm genes and UC.
- To identify novel biomarkers associated with immune microenvironment alterations in UC patients.
Main Methods:
- Analysis of gene expression data from UC patients and controls.
- Identification of differentially expressed genes (DEGs) and functional enrichment.
- Machine learning for biomarker refinement and validation in a mouse colitis model.
Main Results:
- Identified 805 DEGs, including 15 circadian rhythm genes.
- Screened four key genes; PPARGC1A and SLC6A4 validated as hub genes.
- These hub genes correlated with immune cell infiltration in UC.
Conclusions:
- Circadian rhythm gene disruptions are associated with UC pathogenesis.
- PPARGC1A and SLC6A4 show potential as diagnostic biomarkers for UC.
- These findings offer insights into UC's immune microenvironment and potential therapeutic targets.
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