Dickeya ananatis sp. nov., pectinolytic bacterium isolated from pineapple (Ananas comosus)
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View abstract on PubMed
Summary
This summary is machine-generated.A new bacterial species, Dickeya ananatis, is proposed for strains causing pineapple heart rot. Genomic and phylogenetic analyses confirm its distinctness from Dickeya zeae complex, revealing a unique nitrogen fixation gene cluster.
Area Of Science
- Bacteriology and Plant Pathology
- Genomics and Phylogenetics
- Microbial Taxonomy
Background
- The bacterial species Dickeya zeae exhibits complex intraspecies clustering, with some strains potentially representing novel species.
- Bacterial heart rot, caused by Dickeya species, affects pineapple (Ananas comosus) production, necessitating accurate taxonomic identification of causal agents.
Purpose Of The Study
- To genomically characterize and taxonomically classify Dickeya strains isolated from pineapple plants exhibiting bacterial heart rot in Hawaii and Malaysia.
- To determine if these strains represent a distinct species within the Dickeya genus.
Main Methods
- Whole-genome sequencing of seven Dickeya strains isolated from pineapple.
- Comparative genomic analyses using average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH).
- Phylogenetic analyses incorporating 16S rRNA, gapA, multi-locus sequence analysis (MLSA) of ten housekeeping genes, and whole-genome/pangenome comparisons.
- Identification and characterization of specific gene clusters, including nitrogen fixation genes.
Main Results
- Genomic data (ANI and dDDH) indicated that the seven pineapple-derived strains belong to a single species but are distinct from known Dickeya zeae, Dickeya oryzae, and Dickeya parazeae type strains.
- Phylogenetic analyses consistently placed these strains in a separate monophyletic clade, with Dickeya oryzae as the closest relative.
- A novel 28-gene nitrogen fixation gene cluster, similar to that found in Klebsiella spp., was identified in all seven pineapple strains.
Conclusions
- The polyphasic evidence supports the delineation of a new bacterial species, Dickeya ananatis sp. nov., for the pineapple-associated strains.
- The proposed species is distinct from other members of the Dickeya zeae complex.
- The presence of a nitrogen fixation gene cluster may indicate unique metabolic capabilities relevant to the host plant interaction.
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