In vitro assay for erythropoietin

An in vitro assay method for the quantitative estimation of erythropoietin has been developed. The principle of the method is based on the stimulation of LP catalyzed iodination of MIT by erythropoietin and the iodination reaction was found to be linearly proportional to the increasing concentration of erythropoietin of same potency or same concentration of increasing potency. This assay procedure could be utilized for clinical purposes for the estimation of erythropoietin content of biological samples, like urine, serum etc. This method is very simple, rapid, highly specific and sensitive enough to detect as little as 0.25 mU of erythropoietin.