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Bioluminescent Imaging of Patient-Derived Brain Tumors in Live Mice

  • 0Centre for Discovery in Cancer Research, McMaster University, Hamilton, ON, Canada.
Methods in Molecular Biology +

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June 24, 2025

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June 24, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Patient-derived tumor xenograft (PDX) models are vital for brain tumor research. This study details using bioluminescence imaging with the IVIS system to track tumor growth in PDX models non-invasively.

Area Of Science

  • Neuro-oncology
  • Pre-clinical cancer research
  • Biomedical imaging

Background

  • Patient-derived tumor xenograft (PDX) models are essential for studying brain tumor progression and testing new therapies.
  • Non-invasive imaging techniques are needed to monitor tumor development over time.

Purpose Of The Study

  • To present a methodology for analyzing tumor dynamics in PDX brain tumor models.
  • To demonstrate the utility of bioluminescence imaging for quantitative assessment of tumor growth.

Main Methods

  • Utilizing luciferase-expressing PDX brain tumor models.
  • Employing the IVIS® (in vivo imaging) system for bioluminescence imaging.
  • Quantifying tumor growth and dynamics non-invasively across multiple time points.

Main Results

  • Bioluminescence imaging provides a reliable method for tracking tumor progression in PDX models.
  • The IVIS system allows for non-invasive, quantitative analysis of tumor dynamics.
  • This approach facilitates longitudinal studies of brain tumor development.

Conclusions

  • The described methodology enables effective monitoring of brain tumor progression in PDX models.
  • Bioluminescence imaging with the IVIS system is a valuable tool for pre-clinical research.
  • This technique supports the evaluation of therapeutic interventions in brain cancer models.

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