Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

7.0K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
7.0K
Peptide Bonds02:43

Peptide Bonds

77.5K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
77.5K
Protein-protein Interfaces02:04

Protein-protein Interfaces

13.7K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
13.7K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.0K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advanced Degradation and Remediation Strategies for Per- and Polyfluoroalkyl Substances (PFASs): Challenges and Future Perspectives.

Toxics·2026
Same author

Preoperative prediction of thymoma risk classification with machine learning-based computed tomography radiomics features.

World journal of surgical oncology·2026
Same author

High-sensitivity optical thermometry in CaWO<sub>4</sub>: Bi<sup>3+</sup>, Pr<sup>3+</sup> phosphors based on distinct thermal quenching behaviors.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Potassium Biofortification of Quinoa Microgreens: A Holistic Enhancement of Yield, Nutritional Components, and Bioactive Content.

Plant foods for human nutrition (Dordrecht, Netherlands)·2026
Same author

Dual-wavelength LED synergy-tailored charge dynamics and reactive species modulation for a high-performance photocatalytic ceramic membrane reactor in complex water matrices.

Water research·2026
Same author

Adapting federated radiomics models for radiation pneumonitis prediction in patients receiving thoracic radiotherapy with immunotherapy.

Frontiers in immunology·2026
Same journal

Recent advances in Camptothecin-derived antibody-drug conjugates.

Bioorganic & medicinal chemistry·2026
Same journal

CDK4/6-targeted therapy: From clinical inhibitors to emerging strategies to overcome resistance.

Bioorganic & medicinal chemistry·2026
Same journal

Coumarin-sulfonamide hybrids as PKM2 activators induce metabolic reprogramming and suppress ovarian cancer cell growth.

Bioorganic & medicinal chemistry·2026
Same journal

Recent advances in the development of small-molecule drugs based on covalent reversible inhibitors.

Bioorganic & medicinal chemistry·2026
Same journal

Computational hybrid approach of PBPK simulation and CGCNN models for human intravenous pharmacokinetics.

Bioorganic & medicinal chemistry·2026
Same journal

Assessing the use of foundation model embeddings to improve drug response predictions using scRNA-Seq expression data.

Bioorganic & medicinal chemistry·2026
See all related articles

Related Experiment Video

Updated: Sep 18, 2025

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.8K

Tag-like cyclic peptides: Modular affinity elements for protein integration and bioengineering applications.

Zirui Zhu1, Andrew Poweleit2, Louisa Girard3

  • 1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, United States of America; Chemistry Graduate Program, The Ohio State University, Columbus, OH 43210, United States of America.

Bioorganic & Medicinal Chemistry
|June 24, 2025
PubMed
Summary
This summary is machine-generated.

Cyclic peptides offer enhanced stability and specificity for drug development. This review highlights their use as affinity tags to improve protein engineering and therapeutic applications.

Keywords:
Affinity tagsBioengineering applicationsCyclic peptidesDrug developmentProtein engineering

More Related Videos

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.0K
Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.9K

Related Experiment Videos

Last Updated: Sep 18, 2025

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.8K
Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.0K
Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.9K

Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Protein Engineering

Background:

  • Cyclic peptides possess superior stability and specificity compared to linear peptides.
  • They are valuable in therapeutics (antimicrobial, anticancer) and protein engineering as affinity tags.
  • Their unique properties make them promising for advanced drug development.

Purpose of the Study:

  • To provide a comprehensive review of cyclic peptide types and synthesis.
  • To highlight advances in cyclic peptide-based therapeutics, focusing on affinity tag applications.
  • To explore the potential of cyclic peptides in protein engineering and biopharmaceutical development.

Main Methods:

  • Review of existing literature on cyclic peptide synthesis and applications.
  • Exploration of cyclization techniques (disulfide, metal-mediated, organic reagents).
  • Discussion of display technologies (phage, mRNA) for cyclic peptide discovery.

Main Results:

  • Cyclic peptides exhibit enhanced pharmacokinetic and stability profiles.
  • "Tag-like" cyclic peptides can improve protein bioactivity, targeting, and purification.
  • Advanced display technologies enable rapid identification of high-affinity cyclic peptide candidates.

Conclusions:

  • Cyclic peptides are versatile tools in drug development and bioengineering.
  • Their application as affinity tags bridges the gap between small molecules and biologics.
  • Future research directions include optimizing cyclic peptides for protein purification, detection, and interaction studies.