A reaction based fluorescent sensor for the detection of histamine and its application in fish samples

Histamine (His) is responsible for the toxicity related to seafood consumption.However, standard detection methods do not currently meet rapid His analysis requirements. In this study, we developed an anthracene-based fluorescent sensor synthesized from the reaction of3-chloroisonicotinaldehyde and anthracen-9-ylmethylene-hydrazine. UV-Vis and fluorescence spectroscopy were used to determine the selectivity of the sensor in the existence of a range of biogenic amines. FZ-2 showed high sensitivity and selectivity towards His compared to various other biogenic amines in acetonitrile (ACN)/Acetate buffer (10 mM, pH 6.5, v/v, 1:1).The specific response to His was obtained by the substitution reaction of the primary amine of His with chlorine in the pyridine moiety of the sensor. A linear relationship was found between His concentration and fluorescence response of FZ-2 within the range 3.0-50.0 μM. The detection limit was calculated as 2.19 μM. Moreover, FZ-2 was able to monitor His in fish samples using fluorescence spectroscopy.